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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Effects of bacterial vaginosis-associated bacteria and sexual intercourse on vaginal colonization with the probiotic lactobacillus crispatus CTV-05
Sexually Transmitted Diseases, Volume 38, No. 11, Year 2011
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Description
Objective: Several fastidious bacteria have been associated with bacterial vaginosis (BV), but their role in lactobacilli recolonization failure is unknown. We studied the effect of 7 BV-associated bacterial species and 2 Lactobacillus species on vaginal colonization with Lactobacillus crispatus CTV-05 (LACTIN-V). Methods: Twenty-four women with BV were given a 5-day course of metronidazole vaginal gel and then randomized 3:1 to receive either LACTIN-V or placebo applied vaginally once daily for 5 initial consecutive days, followed by a weekly application over 2 weeks. Vaginal swabs for L. crispatus CTV-05 culture and 9 bacterium-specific 16S rRNA gene quantitative polymerase chain reaction assays were analyzed on several study visits for the 18 women receiving LACTIN-V. Results: Vaginal colonization with CTV-05 was achieved in 61% of the participants receiving LACTIN-V at either day 10 or day 28 visit and 44% at day 28. Participants not colonized with CTV-05 had generally higher median concentrations of BV-associated bacteria compared to those who colonized. Between enrollment and day 28, the median concentration of Gardnerella vaginalis minimally reduced from 10 to 10 16S rRNA gene copies per swab in women who colonized with CTV-05 but increased from 10 to 10 in those who failed to colonize (P = 0.19). Similarly, the median concentration of Atopobium spp. reduced from 10 16S rRNA gene copies per swab to below limit of detection in women who colonized with CTV-05, but increased from 10 to 10 in those who failed to colonize (P = 0.04). The presence of endogenous L. crispatus at enrollment was found to be significantly associated with a reduced odds of colonization with CTV-05 on day 28 (P = 0.003), and vaginal intercourse during the study significantly impaired successful CTV-05 colonization (P = 0.018). Conclusion: Vaginal concentration of certain BV-associated bacteria, vaginal intercourse during treatment, and the presence of endogenous L. crispatus at enrollment predict colonization with probiotic lactobacilli. © 2011 American Sexually Transmitted Diseases Association All rights reserved.
Authors & Co-Authors
Ngugi, Benjamin
Kenya, Nairobi
Kenya Medical Research Institute
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Hemmerling, Anke
United States, San Francisco
University of California, San Francisco
Bukusi, Elizabeth Anne
Kenya, Nairobi
Kenya Medical Research Institute
Kikuvi, Gideon Mutie
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
United States, Seattle
University of Washington
Gikunju, Joseph Kangangi
Kenya, Nairobi
Jomo Kenyatta University of Agriculture and Technology
Shiboski, Stephen C.
United States, San Francisco
University of California, San Francisco
Fredricks, David N.
United States, Seattle
Fred Hutchinson Cancer Research Center
United States, Seattle
University of Washington
Cohen, Craig R.
United States, San Francisco
University of California, San Francisco
Statistics
Citations: 56
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1097/OLQ.0b013e3182267ac4
ISSN:
01485717
e-ISSN:
15374521
Research Areas
Genetics And Genomics
Sexual And Reproductive Health
Study Approach
Quantitative
Participants Gender
Female