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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Rapid Aeromonas hydrophila identification by TaqMan PCR assay: Comparison with a phenotypic method
Letters in Applied Microbiology, Volume 49, No. 2, Year 2009
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Description
Aims: Aeromonas hydrophila is recognized as a human pathogen following wound exposure or ingestion of contaminated water and food. For rapid identification of this bacterium, a TaqMan-based real-time PCR assay has been developed. Methods and Results: Primers and probes that target specific sequences of the 16S rRNA gene and cytolytic enterotoxin gene (aerA) were combined in a duplex assay. Presence and size of PCR products were confirmed with microchannel fluidics electrophoresis analysis. After validation, using type strain CIP7614T DNA, the PCR assay was tested on 12 positive and negative controls. Twenty-one Aeromonas strains were isolated from environmental samples and were identified with biochemical tests as Aer. sobria, Aer. caviae and Aer. hydrophila. Only Aer. hydrophila strains tested positive by PCR assay. Conclusions: The PCR developed here was successfully applied for the identification of Aer. hydrophila from reference, clinical and environmental samples and showed a high discrimination between Aer. hydrophila and other Aeromonas species. Significance and Impact of the Study: This molecular method is convenient, rapid (2·5 h vs 24 h), specific to identify Aer. hydrophila and usable for diagnosis in medical and veterinary laboratories. © 2009 The Authors.
Authors & Co-Authors
Trakhna, Faten
France, Beauvais
Institut Polytechnique Lasalle Beauvais
Tunisia, Tunis
Institut Pasteur de Tunis
Tunisia, Tunis
University of Carthage, Institut National Agronomique de Tunisie
Harf-Monteil, Colette
France, Strasbourg
Université de Strasbourg
Abdel Nour, Afif M.
France, Beauvais
Institut Polytechnique Lasalle Beauvais
Maaroufi, Abderrazek
Tunisia, Tunis
Institut Pasteur de Tunis
Gadonna-Widehem, Pascale
France, Beauvais
Institut Polytechnique Lasalle Beauvais
Statistics
Citations: 39
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1111/j.1472-765X.2009.02635.x
ISSN:
02668254
e-ISSN:
1472765X
Research Areas
Environmental
Food Security
Genetics And Genomics