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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling
Journal of Virology, Volume 86, No. 8, Year 2012
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Description
Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. To this end we produced HSV-1 and -2 proteome microarrays in Escherichia coli and probed them against a panel of sera from patients serotyped using commercial gG-1 and gG-2 (gGs for HSV-1 and -2, respectively) enzyme-linked immunosorbent assays. We identified many reactive antigens in both HSV-1 and -2, some of which were type specific (i.e., recognized by HSV-1- or HSV-2-positive donors only) and others of which were nonspecific or cross-reactive (i.e., recognized by both HSV-1- and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli. © 2012, American Society for Microbiology.
Authors & Co-Authors
Kalantari, Mina
United States, Irvine
Uci School of Medicine
Chun, Sookhee
United States, Irvine
Uci School of Medicine
Chentoufi, Aziz Alami
United States, Irvine
Uci School of Medicine
Saudi Arabia, Riyadh
King Fahad Medical City
Pablo, Jozelyn
United States, Irvine
Uci School of Medicine
United States, Irvine
Antigen Discovery Inc.
Liang, Li
United States, Irvine
Uci School of Medicine
Dasgupt, Gargi
United States, Irvine
Uci School of Medicine
Molina, Douglas M.
United States, Irvine
Antigen Discovery Inc.
Jasinskas, Algis
United States, Irvine
Uci School of Medicine
Nakajima, Rie
United States, Irvine
Uci School of Medicine
Felgner, Jiin H.
United States, Irvine
Antigen Discovery Inc.
Hermanson, Gary
United States, Irvine
Antigen Discovery Inc.
BenMohamed, Lbachir
United States, Irvine
Uci School of Medicine
Felgner, Philip Louis
United States, Irvine
Uci School of Medicine
Davies, David Huw
United States, Irvine
Uci School of Medicine
United States, Irvine
Antigen Discovery Inc.
Statistics
Citations: 47
Authors: 14
Affiliations: 3
Identifiers
Doi:
10.1128/JVI.05194-11
ISSN:
0022538X
e-ISSN:
10985514
Research Areas
Environmental