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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale
Genomics, Volume 90, No. 6, Year 2007
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Description
The coevolution of ticks and the pathogens that they transmit has ensured their mutual survival. In these studies, we used a functional genomics approach to characterize tick genes regulated in response to Anaplasma marginale infection. Differentially regulated genes/proteins were identified by suppression-subtractive hybridization and differential in-gel electrophoresis analyses of cultured IDE8 tick cells infected with A. marginale. Nine of 17 of these genes were confirmed by real-time RT-PCR to be differentially regulated in ticks and/or IDE8 tick cells in response to A. marginale infection. RNA interference was used for functional studies. Six genes, which encode putative selenoprotein W2a, hematopoietic stem/progenitor cells protein-like, proteasome 26S subunit, ferritin, GST, and subolesin control, were found to affect A. marginale infection in IDE8 tick cells. Four genes, which encode putative GST, salivary selenoprotein M, vATPase, and ubiquitin, affected A. marginale infection in different sites of development in ticks. The results of these studies demonstrated that a molecular mechanism occurs by which tick cell gene expression mediates the A. marginale developmental cycle and trafficking through ticks. © 2007 Elsevier Inc. All rights reserved.
Authors & Co-Authors
de la Fuente, José Luis
United States, Stillwater
Oklahoma State University
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Blouin, Edmour F.
United States, Stillwater
Oklahoma State University
Manzano-Román, Raúl
United States, Stillwater
Oklahoma State University
Naranjo, Victoria
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Almazán, Consuelo
Mexico, Ciudad Victoria
Universidad Autónoma de Tamaulipas
Pérez de la Lastra, José Manuel
Spain, Ciudad Real
Csic-uclm - Instituto de Investigacion en Recursos Cinegeticos Irec
Zivkovic, Zorica
Netherlands, Utrecht
Afdeling Infectieziekten en Immunologie
Jongejan, Frans
Netherlands, Utrecht
Afdeling Infectieziekten en Immunologie
South Africa, Pretoria
University of Pretoria
Kocan, Katherine M.
United States, Stillwater
Oklahoma State University
Statistics
Citations: 93
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.ygeno.2007.08.009
ISSN:
08887543
e-ISSN:
10898646
Research Areas
Cancer
Genetics And Genomics