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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Efficiency of the polymerase chain reaction
Chemical Engineering Science, Volume 65, No. 17, Year 2010
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Description
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly, quantitative real time PCR is used to assess copy numbers from overall yield. In this study the yield is analyzed as a function of several processes: (1) thermal damage of the template and polymerase occurring during the denaturing step, (2) competition existing between primers and templates to either anneal or form dsDNA, (3) polymerase binding to annealed products (primer/ssDNA) to form ternary complexes and (4) extension of ternary complexes. Explicit expressions are provided for the efficiency of each process, therefore reaction conditions can be directly linked to the overall yield. Examples are provided where different processes play the yield-limiting role. The analysis will give researchers a unique understanding of the factors that control the reaction and will aid in the interpretation of experimental results. © 2010 Elsevier Ltd.
Authors & Co-Authors
Booth, Christine S.
United States, Lincoln
University of Nebraska–lincoln
Pienaar, Elsje
United States, Lincoln
University of Nebraska–lincoln
TerMaat, Joel R.
United States, Lincoln
University of Nebraska–lincoln
Whitney, Scott E.
United States, Lincoln
University of Nebraska–lincoln
Louw, Tobias M.
United States, Lincoln
University of Nebraska–lincoln
Viljoen, Hendrik J.
United States, Lincoln
University of Nebraska–lincoln
Statistics
Citations: 25
Authors: 6
Affiliations: 1
Identifiers
Doi:
10.1016/j.ces.2010.05.046
ISSN:
00092509
Research Areas
Cancer
Genetics And Genomics
Study Approach
Quantitative