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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Kaposi's sarcoma-associated herpesvirus serology in Europe and Uganda: Multicentre study with multiple and novel assays
Journal of Medical Virology, Volume 65, No. 1, Year 2001
Notification
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Description
A multicentre study was undertaken to define novel assays with increased inter-assay concordance, sensitivity, specificity and predictive value for serological diagnosis of human herpesvirus type 8 (HHV-8) infection. A total of 562 sera from European and Ugandan human immunodeficiency virus (HIV)-infected or uninfected individuals with or without Kaposi's sarcoma (KS) and blood donors were examined under code by 18 different assays in seven European laboratories. Sera from KS patients and all non-KS sera found positive by at least 70%, 80%, or 90% of the assays were considered "true positive." The validity of the assays was then evaluated by univariate logistic regression analysis. Two immunofluorescence assays (IFA) for detection of antibodies against HHV-8 lytic (Rlyt) or latent (LLANA) antigens and two enzyme-linked-immunosorbent assays (ELISA) (M2, EK8.1) for detection of antibodies against HHV-8 structural proteins were found to be highly concordant, specific, and sensitive, with odds ratios that indicated a high predictive value. When used together, the two IFA (Rlyt-LLANA) showed the best combination of sensitivity (89.1%) and specificity (94.9%). The performance of these assays indicate that they may be used for the clinical management of individuals at risk of developing HHV-8 associated tumours such as allograft recipients. © 2001 Wiley-Liss, Inc.
Authors & Co-Authors
Schatz, Octavian
Germany, Munich
Ludwig-maximilians-universität München
Monini, P.
Italy, Rome
Istituto Superiore Di Sanita
Bugarini, Roberto
Italy, Rome
Istituto Superiore Di Sanita
Neipel, Frank
Germany, Erlangen
Friedrich-alexander-universität Erlangen-nürnberg
Schulz, Thomas Friedrich
United Kingdom, Liverpool
University of Liverpool
Andreoni, Massimo G.
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Erb, Peter
Switzerland, Basel
University of Basel, Institute for Medical Microbiology
Eggers, Maren
Germany, Stuttgart
Medizin-diagnost Gemeinschaftslabor
Haas, Jürgen G.
Germany, Munich
Ludwig-maximilians-universität München
Buttò, Stefano
Italy, Rome
Istituto Superiore Di Sanita
Lukwiya, Matthew
Uganda, Gulu
St. Mary's Hospital Lacor - Uganda
Bogner, Johannes Richard
Germany, Munich
Ludwig-maximilians-universität München
Yaguboglu, Svenja
Germany, Erlangen
Friedrich-alexander-universität Erlangen-nürnberg
Sheldon, Julie A.
United Kingdom, Liverpool
University of Liverpool
Sarmati, Loredana
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Goebel, Frank Detlef
Germany, Munich
Ludwig-maximilians-universität München
Hintermaier, Renate
Germany, Munich
Ludwig-maximilians-universität München
Enders, Gisela
Germany, Stuttgart
Medizin-diagnost Gemeinschaftslabor
Regamey, Nicolas
Switzerland, Basel
University of Basel, Institute for Medical Microbiology
Wernli, Marion
Switzerland, Basel
University of Basel, Institute for Medical Microbiology
Stürzl, Michael
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Rezza, Giovanni
Italy, Rome
Istituto Superiore Di Sanita
Ensoli, Barbara
Italy, Rome
Istituto Superiore Di Sanita
Statistics
Citations: 72
Authors: 23
Affiliations: 9
Identifiers
Doi:
10.1002/jmv.2011
ISSN:
01466615
Research Areas
Cancer
Infectious Diseases
Study Approach
Quantitative
Study Locations
Uganda