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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Isolation and detection of circulating tumour cells from metastatic melanoma patients using a slanted spiral microfluidic device
Oncotarget, Volume 8, No. 40, Year 2017
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Description
Circulating Tumour Cells (CTCs) are promising cancer biomarkers. Several methods have been developed to isolate CTCs from blood samples. However, the isolation of melanoma CTCs is very challenging as a result of their extraordinary heterogeneity, which has hindered their biological and clinical study. Thus, methods that isolate CTCs based on their physical properties, rather than surface marker expression, such as microfluidic devices, are greatly needed in melanoma. Here, we assessed the ability of the slanted spiral microfluidic device to isolate melanoma CTCs via label-free enrichment. We demonstrated that this device yields recovery rates of spiked melanoma cells of over 80% and 55%, after one or two rounds of enrichment, respectively. Concurrently, a two to three log reduction of white blood cells was achieved with one or two rounds of enrichment, respectively. We characterised the isolated CTCs using multimarker flow cytometry, immunocytochemistry and gene expression. The results demonstrated that CTCs from metastatic melanoma patients were highly heterogeneous and commonly expressed stem-like markers such as PAX3 and ABCB5. The implementation of the slanted microfluidic device for melanoma CTC isolation enables further understanding of the biology of melanoma metastasis for biomarker development and to inform future treatment approaches. © 2017 Impact Journals LLC. All rights reserved.
Authors & Co-Authors
Khattak, Muhammad Adnan
Australia, Perth
Fiona Stanley Hospital
Australia, Perth
The University of Western Australia
Warkiani, Majid Ebrahimi
Australia, Sydney
Unsw Sydney
Gray, Elin Solomonovna
Australia, Perth
Edith Cowan University
Ziman, Mel
Australia, Perth
Edith Cowan University
Australia, Perth
The University of Western Australia
Statistics
Citations: 41
Authors: 4
Affiliations: 8
Identifiers
Doi:
10.18632/ONCOTARGET.18641
ISSN:
19492553
Research Areas
Cancer
Genetics And Genomics