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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering
Materials Science and Engineering C, Volume 30, No. 8, Year 2010
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Description
Surface properties of scaffolds such as hydrophilicity and the presence of functional groups on the surface of scaffolds play a key role in cell adhesion, proliferation and migration. Different modification methods for hydrophilicity improvement and introduction of functional groups on the surface of scaffolds have been carried out on synthetic biodegradable polymers, for tissue engineering applications. In this study, alkaline hydrolysis of poly (ε-caprolactone) (PCL) nanofibrous scaffolds was carried out for different time periods (1 h, 4 h and 12 h) to increase the hydrophilicity of the scaffolds. The formation of reactive groups resulting from alkaline hydrolysis provides opportunities for further surface functionalization of PCL nanofibrous scaffolds. Matrigel was attached covalently on the surface of an optimized 4 h hydrolyzed PCL nanofibrous scaffolds and additionally the fabrication of blended PCL/matrigel nanofibrous scaffolds was carried out. Chemical and mechanical characterization of nanofibrous scaffolds were evaluated using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, contact angle, scanning electron microscopy (SEM) and tensile measurement. In vitro cell adhesion and proliferation study was carried out after seeding nerve precursor cells (NPCs) on different scaffolds. Results of cell proliferation assay and SEM studies showed that the covalently functionalized PCL/matrigel nanofibrous scaffolds promote the proliferation and neurite outgrowth of NPCs compared to PCL and hydrolyzed PCL nanofibrous scaffolds, providing suitable substrates for nerve tissue engineering. © 2010 Elsevier B.V.
Authors & Co-Authors
Ghasemi-Mobarakeh, Laleh
Singapore, Singapore City
National University of Singapore
Iran, Najafabad
Islamic Azad University, Najafabad Branch
Iran, Isfahan
Isfahan University of Technology
Iran, Tehran
Royan Institute
Prabhakaran, Molamma P.
Singapore, Singapore City
National University of Singapore
Morshed, Mohammad
Iran, Isfahan
Isfahan University of Technology
Nasr-Esfahani, Mohammad Hossein
Iran, Tehran
Royan Institute
Ramakrishna, Seeram A.
Singapore, Singapore City
National University of Singapore
Saudi Arabia, Riyadh
King Saud University
Statistics
Citations: 182
Authors: 5
Affiliations: 5
Identifiers
Doi:
10.1016/j.msec.2010.06.004
ISSN:
09284931