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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Genetic characterisation of CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli strains isolated from stem cell transplant patients in Tunisia
International Journal of Antimicrobial Agents, Volume 32, No. 4, Year 2008
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Description
Characterisation of extended-spectrum β-lactamase (ESBL) genes and their genetic environments as well as the presence of integrons were analysed in nine Klebsiella pneumoniae and two Escherichia coli ESBL-positive isolates recovered in the Centre of Bone Marrow Transplantation of Tunisia. All strains harboured the blaCTX-M-15 gene and presented minimum inhibitory concentrations for cefotaxime and ceftazidime of 256-1024 mg L-1 and 16-512 mg L-1, respectively, and eight of them showed different pulsed-field gel electrophoresis patterns. The blaOXA-1 and blaTEM-1 genes were detected in eight and ten strains, respectively. In addition, blaSHV-1, blaSHV-11 and blaSHV-27 were found in six, one and one K. pneumoniae strains, respectively. The new variant blaSHV-103 was characterised in one K. pneumoniae strain. The intI1 gene was detected in eight K. pneumoniae strains and the dfrA5 + ereA2 and aadA gene cassettes were found in one and five strains, respectively. All strains harboured a 70 kb plasmid, and its transference in addition to blaCTX-M-15, blaTEM-1b and blaOXA-1 genes was demonstrated from three K. pneumoniae to E. coli. ISEcp1 and orf477 were located upstream and downstream, respectively, of the blaCTX-M-15 gene in 10 strains. The occurrence of the blaCTX-M-15 gene in unrelated strains might have originated from the dissemination of mobile genetic elements in which ISEcp1 may have played an important role. © 2008 Elsevier B.V. and the International Society of Chemotherapy.
Authors & Co-Authors
Abbassi, Mohamed Salah
Tunisia, Tunis
Centre National de Greffe de Moelle Osseuse
Torres, Carmen
Spain, Logrono
Universidad de la Rioja
Achour, W.
Tunisia, Tunis
Centre National de Greffe de Moelle Osseuse
Vinué, Laura
Spain, Logrono
Universidad de la Rioja
Sáenz, Yolanda
Spain, Logrono
Universidad de la Rioja
Costa, Daniela
Spain, Logrono
Universidad de la Rioja
Bouchami, Ons
Tunisia, Tunis
Centre National de Greffe de Moelle Osseuse
Ben Hassen, Assia
Tunisia, Tunis
Centre National de Greffe de Moelle Osseuse
Statistics
Citations: 65
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijantimicag.2008.04.009
ISSN:
09248579
Research Areas
Cancer
Genetics And Genomics
Study Locations
Tunisia