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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Distribution, antioxidant and characterisation of phenolic compounds in soybeans, flaxseed and olives
Food Chemistry, Volume 139, No. 1-4, Year 2013
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Description
The distribution of free and bound phenolic compounds present in soybean, flaxseed and olive were investigated. The phenolic compounds were fractionated on the basis on their solubility characteristics in water, alcohol, dilute base and dilute acid. Reversed phase high pressure liquid chromatography (RP-HPLC) and mass spectrometry (MS) were used for identification of individual components of phenolic compounds. Antioxidant activity (AA%) of free and bound phenolic compounds was measured using the linoleic acid/β-carotene assay. The water-soluble phenolic compound fractions represented 68-81%, 50-72% and 46-56% of the total phenolic compounds measured in full-fat soybean, olive and flaxseed, respectively. Methanolic extraction of free phenolic compounds without heat, solubilised 21-56%, 42-62% and 34-51% of the total phenolic compounds measured in soybean, olive and flaxseed, respectively; methanol extraction of free phenolic compounds with heat solubilised a further 24-34%, 31-37% and 36-37% of phenolic compounds from soybean, olive and flaxseed, respectively. Further dilute alkali and dilute acid solubilised the remaining 10-40%, 1-21% and 12-29% of the total phenolic compounds from soybean, olive and flaxseed, respectively. Results indicated that the full-fat meals of soybean, flaxseed and olive showed higher antioxidant activity compared to defatted meals. RP-HPLC and LC-MS/MS profil1 for soybean, flaxseed and olive indicate two classes of phenolic compounds designated as free and bound phenolic compounds. © 2013 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Rababah, Taha M.
Jordan, Irbid
Jordan University of Science and Technology
Ereifej, Khalil I.
Jordan, Irbid
Jordan University of Science and Technology
Alli, Inteaz
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Statistics
Citations: 102
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1016/j.foodchem.2012.12.061
ISSN:
03088146
e-ISSN:
18737072
Research Areas
Environmental
Substance Abuse