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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
The isolation of RNA from raspberry (Rubus idaeus) fruit
Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology, Volume 8, No. 3, Year 1997
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Description
Previous attempts to extract high-quality, total RNA from raspberry (Rubus idaeus) fruits using published protocols have proven to be unsuccessful. Even the use of protocols developed for the extraction of RNA from other fruit tissue has resulted in low yields (1) or the isolation of degraded RNA (2). Here, we report on the development of a quick and simple method of extracting total RNA from raspberry fruit. Using this method, high yields of good quality, undegraded RNA were obtained from fruit at all stages of ripening. The RNA is of sufficient quality for northern analysis and cDNA library construction. © 1997 Humana Press Inc. All rights of any nature whatsoever reserved.
Authors & Co-Authors
Jones, Chris Stephen
United Kingdom, Aberdeen
The James Hutton Institute
Iannetta, P. P.M.
United Kingdom, Aberdeen
The James Hutton Institute
Davies, H. V.
United Kingdom, Aberdeen
The James Hutton Institute
McNicol, Ronnie J.
United Kingdom, Aberdeen
The James Hutton Institute
Taylor, Mark A.
United Kingdom, Aberdeen
The James Hutton Institute
Statistics
Citations: 27
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1007/BF02760775
ISSN:
10736085
Research Areas
Genetics And Genomics