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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of the Determine™ fourth generation HIV rapid assay
Journal of Virological Methods, Volume 189, No. 1, Year 2013
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Description
Assays that detect p24 antigen reduce the diagnostic window period of HIV testing. Most point-of-care HIV assays have poor sensitivity to diagnose acute HIV infection as they only detect antibodies against HIV-1 and HIV-2 (HIV-1/2). This was a cross-sectional laboratory-based study that evaluated the performance of the Determine™ HIV-1/2 Ag/Ab Combo fourth generation rapid strip - currently the only rapid assay that detects both HIV-1/2 antibodies and p24 antigen. A total of 79 serum specimens (29 positive for HIV antibodies only, 14 positive for HIV antibodies and p24 antigen, 20 HIV-negative, and 16 positive for p24 antigen only) were used for the evaluation. Results were compared with those from validated fourth generation HIV ELISAs. The Determine™ Combo rapid strips had a sensitivity of 90.7% and a specificity of 100% for the detection of HIV-1/2 antibodies. Its sensitivity for the detection of p24 antigen was only 10% (3 out of 30 p24 antigen positive specimens). This implies that most acute HIV infections will be missed with this assay. The need for a point-of-care assay which can detect acute HIV infection reliably still remains, particularly for use in a high prevalence setting such as South Africa. © 2013 Elsevier B.V.
Authors & Co-Authors
Brauer, Marieke
South Africa, Pretoria
University of Pretoria
De Villiers, Johanna C.
South Africa, Pretoria
University of Pretoria
Mayaphi, Simnikiwe H.
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 33
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1016/j.jviromet.2013.01.017
ISSN:
01660934
e-ISSN:
18790984
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
South Africa