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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Biomphalaria glabrata peroxiredoxin: Effect of Schistosoma mansoni infection on differential gene regulation
Molecular and Biochemical Parasitology, Volume 167, No. 1, Year 2009
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Description
To identify gene(s) that may be associated with resistance/susceptibility in the intermediate snail host Biomphalaria glabrata to Schistosoma mansoni infection, a snail albumen gland cDNA library was differentially screened and a partial cDNA encoding an antioxidant enzyme thioredoxin peroxidase (Tpx), or peroxiredoxin (Prx), was identified. The 753 bp full-length, single-copy, constitutively expressed gene now referred to as BgPrx4 was later isolated. BgPrx4 is a 2-Cys peroxiredoxin containing the conserved peroxidatic cysteine (CP) in the N-terminus and the resolving cysteine (CR) in the C-terminus. Sequence analysis of BgPrx4 from both resistant and susceptible snails revealed the presence of several (at least 7) single nucleotide polymorphisms (SNPs). Phylogenetic analysis indicated BgPrx4 to resemble a homolog of human peroxiredoxin, PRDX4. Northern analysis of hepatopancreas RNA from both resistant and susceptible snails showed that upon parasite exposure there were qualitative changes in gene expression. Quantitative real-time RT-PCR analysis showed differences in the levels of BgPrx4 transcript induction following infection, with the transcript up-regulated in resistant snails during the early phase (5 h) of infection compared to susceptible snails in which it was down-regulated within the early time period. While there was an increase in transcription in susceptible snails later (48 h) post-infection, this never reached the levels detected in resistant snails. A similar trend - higher, earlier up-regulation in the resistant snails but lower, slower protein expression in susceptible snails - was observed by Western blot analysis. Enzymatic analysis of the purified, recombinant BgPrx4 revealed the snail sequence to function as Prx but with an unusual ability to use both thioredoxin and glutathione as substrates. © 2009 Elsevier B.V.
Authors & Co-Authors
Knight, Matty
United States, Rockville
Biomedical Research Institute, Rockville
Raghavan, Nithya
United States, Rockville
Biomedical Research Institute, Rockville
Goodall, Cheri
United States, Corvallis
Oregon State University
Cousin, Carolyn
United States, Washington, D.c.
University of the District of Columbia
Ittiprasert, Wannaporn
United States, Rockville
Biomedical Research Institute, Rockville
Sayed, Ahmed Abdel Aziz
Egypt, Cairo
College of Science
Miller, Andre
United States, Rockville
Biomedical Research Institute, Rockville
Williams, David Lee
United States, Chicago
Rush University Medical Center
Bayne, Christopher J.
United States, Corvallis
Oregon State University
Statistics
Citations: 26
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.molbiopara.2009.04.002
ISSN:
01666851
Research Areas
Genetics And Genomics
Study Approach
Qualitative
Quantitative