Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
The Immunological and Virological Consequences of Planned Treatment Interruptions in Children with HIV Infection
PLoS ONE, Volume 8, No. 10, Article e76582, Year 2013
Notification
URL copied to clipboard!
Description
Objectives:To evaluate the immunological and viral consequences of planned treatment interruptions (PTI) in children with HIV.Design:This was an immunological and virological sub-study of the Paediatric European Network for Treatment of AIDS (PENTA) 11 trial, which compared CD4-guided PTI of antiretroviral therapy (ART) with continuous therapy (CT) in children.Methods:HIV-1 RNA and lymphocyte subsets, including CD4 and CD8 cells, were quantified on fresh samples collected during the study; CD45RA, CD45RO and CD31 subpopulations were evaluated in some centres. For 36 (18 PTI, 18 CT) children, immunophenotyping was performed and cell-associated HIV-1 DNA analysed on stored samples to 48 weeks.Results:In the PTI group, CD4 cell count fell rapidly in the first 12 weeks off ART, with decreases in both naïve and memory cells. However, the proportion of CD4 cells expressing CD45RA and CD45RO remained constant in both groups. The increase in CD8 cells in the first 12 weeks off ART in the PTI group was predominantly due to increases in RO-expressing cells. PTI was associated with a rapid and sustained increase in CD4 cells expressing Ki67 and HLA-DR, and increased levels of HIV-1 DNA.Conclusions:PTI in children is associated with rapid changes in CD4 and CD8 cells, likely due to increased cell turnover and immune activation. However, children off treatment may be able to maintain stable levels of naïve CD4 cells, at least in proportion to the memory cell pool, which may in part explain the observed excellent CD4 cell recovery with re-introduction of ART. © 2013 Klein et al.
Authors & Co-Authors
Klein, Nigel J.
United Kingdom, London
University College London
Zanchetta, Marisa
Italy, Padua
Università Degli Studi Di Padova
Castro, Hannah
United Kingdom, London
Medical Research Council
Bernardi, Stefania
Italy, Rome
Irccs Ospedale Pediatrico Bambino Gesù
Morris, Lynn G.
United Kingdom, London
University College London
Giaquinto, Carlo
Italy, Padua
Università Degli Studi Di Padova
Walker, A. Sarah
United Kingdom, London
Medical Research Council
Gibb, Diana M.
United Kingdom, London
Medical Research Council
De Rossi, Anita
Italy, Padua
Università Degli Studi Di Padova
Statistics
Citations: 32
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0076582
ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases
Maternal And Child Health