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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Human immunodeficiency virus-specific gamma interferon enzyme-linked immunospot assay responses targeting specific regions of the proteome during primary subtype C infection are poor predictors of the course of viremia and set point
Journal of Virology, Volume 83, No. 1, Year 2009
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Description
It is unknown whether patterns of human immunodeficiency virus (HIV)-specific T-cell responses during acute infection may influence the viral set point and the course of disease. We wished to establish whether the magnitude and breadth of HIV type 1 (HIV-1)-specific T-cell responses at 3 months postinfection were correlated with the viral-load set point at 12 months and hypothesized that the magnitude and breadth of HIV-specific T-cell responses during primary infection would predict the set point. Gamma interferon (IFN-γ) enzyme-linked immunospot (ELISPOT) assay responses across the complete proteome were measured in 47 subtype C HIV-1-infected participants at a median of 12 weeks postinfection. When corrected for amino acid length and individuals responding to each region, the order of recognition was as follows: Nef > Gag > Pol > Rev > Vpr > Env > Vpu > Vif > Tat. Nef responses were significantly (P < 0.05) dominant, targeted six epitopic regions, and were unrelated to the course of viremia. There was no significant difference in the magnitude and breadth of responses for each protein region with disease progression, although there was a trend of increased breadth (mean, four to seven pools) in rapid progressors. Correlation of the magnitude and breadth of IFN-γ responses with the viral set point at 12 months revealed almost zero association for each protein region. Taken together, these data demonstrate that the magnitude and breadth of IFN-γ ELISPOT assay responses at 3 months postinfection are unrelated to the course of disease in the first year of infection and are not associated with, and have low predictive power for, the viral set point at 12 months. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Gray, Clive M.
South Africa, Johannesburg
National Institute for Communicable Diseases
Mlotshwa, Mandla
South Africa, Johannesburg
National Institute for Communicable Diseases
Riou, Catherine
South Africa, Johannesburg
National Institute for Communicable Diseases
Mathebula, Tiyani Y.
South Africa, Johannesburg
National Institute for Communicable Diseases
Rosa, Debra De Assis
South Africa, Johannesburg
National Institute for Communicable Diseases
Mashishi, Tumelo N.
South Africa, Johannesburg
National Institute for Communicable Diseases
Seoighe, Cathal
South Africa, Cape Town
University of Cape Town
Ngandu, Nobubelo Kwanele
South Africa, Cape Town
University of Cape Town
van Loggerenberg, Francois
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Mlisana, Koleka P.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Williamson, Carolyn
South Africa, Cape Town
University of Cape Town
Abdool Karim, Salim S.
South Africa, Congella
Centre for the Aids Programme of Research in South Africa
Statistics
Citations: 72
Authors: 13
Affiliations: 3
Identifiers
Doi:
10.1128/JVI.01678-08
ISSN:
0022538X
Research Areas
Infectious Diseases