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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
High incidence of DNA ploidy abnormalities and increased Mcm2 expression may predict malignant change in oral proliferative verrucous leukoplakia
Histopathology, Volume 62, No. 4, Year 2013
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Description
Aims: To assess the DNA content of cases of oral proliferative verrucous leukoplakia (PVL) and correlate the DNA ploidy findings with the expression of Mcm2, geminin, and Ki67, and with clinicopathological data. Methods and results: DNA quantification was performed by image cytometry using the ACIS III Automated Cellular Imaging System. Expression of Ki67, Mcm2 and geminin was determined by immunohistochemistry. There were 21 cases of PVL, the female/male ratio was 6:1, and the average age was 65.5 years. Seventeen patients (81.0%) did not report tobacco and alcohol consumption. Nine patients (42.9%) developed verrucous or squamous cell carcinoma. Levels of Mcm2 expression showed a positive correlation with increasingly severe epithelial changes (P = 0.03). Twenty patients had their DNA examined by ACIS III, and 19 (95%) showed aneuploidy. The frequency and severity of aneuploidy (P < 0.0001), the mean values of the DNA heterogeneity index (P < 0.0001) and the 5n-exceeding fractions (P = 0.0007) increased according to epithelial alterations. Abnormal DNA content was observed even in the more indolent lesions. Conclusions: Mcm2 expression and DNA ploidy analysis could be used to predict areas of malignant transformation. The clinicopathological findings associated with the immunohistochemical and DNA ploidy results support the distinct and aggressive profile of this entity. © 2012 Blackwell Publishing Ltd.
Authors & Co-Authors
Santos-Silva, Alan Roger
Brazil, Campinas
Universidade Estadual de Campinas
Speight, Paul M.
United Kingdom, Sheffield
The University of Sheffield
Hunter, Keith D.
United Kingdom, Sheffield
The University of Sheffield
Carlos-Bregni, Román
Guatemala, Guatemala City
Centro Clínico de Cabeza y Cuello/hospital Herrera Llerandi
Vargas, P. A.
Brazil, Campinas
Universidade Estadual de Campinas
Almeida, Oslei Paes de
Brazil, Campinas
Universidade Estadual de Campinas
Lopes, Márcio Ajudarte
Brazil, Campinas
Universidade Estadual de Campinas
Statistics
Citations: 38
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1111/his.12036
ISSN:
13652559
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Substance Abuse
Study Design
Cohort Study
Participants Gender
Male
Female