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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
In vitro evaluation of a soluble Leishmania promastigote surface antigen as a potential vaccine candidate against human leishmaniasis
PLoS ONE, Volume 9, No. 5, Article e92708, Year 2014
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Description
PSA (Promastigote Surface Antigen) belongs to a family of membrane-bound and secreted proteins present in several Leishmania (L.) species. PSA is recognized by human Th1 cells and provides a high degree of protection in vaccinated mice. We evaluated humoral and cellular immune responses induced by a L. amazonensis PSA protein (LaPSA-38S) produced in a L. tarentolae expression system. This was done in individuals cured of cutaneous leishmaniasis due to L. major (CCLm) or L. braziliensis (CCLb) or visceral leishmaniasis due to L. donovani (CVLd) and in healthy individuals. Healthy individuals were subdivided into immune (HHR-Lm and HHR-Li: Healthy High Responders living in an endemic area for L. major or L. infantum infection) or non immune/naive individuals (HLR: Healthy Low Responders), depending on whether they produce high or low levels of IFN-γ in response to Leishmania soluble antigen. Low levels of total IgG antibodies to LaPSA-38S were detected in sera from the studied groups. Interestingly, LaPSA-38S induced specific and significant levels of IFN-γ, granzyme B and IL-10 in CCLm, HHR-Lm and HHR-Li groups, with HHR-Li group producing TNF-α in more. No significant cytokine response was observed in individuals immune to L. braziliensis or L. donovani infection. Phenotypic analysis showed a significant increase in CD4+ T cells producing IFN-γ after LaPSA-38S stimulation, in CCLm. A high positive correlation was observed between the percentage of IFN-γ-producing CD4+ T cells and the released IFN-γ. We showed that the LaPSA-38S protein was able to induce a mixed Th1 and Th2/Treg cytokine response in individuals with immunity to L. major or L. infantum infection indicating that it may be exploited as a vaccine candidate. We also showed, to our knowledge for the first time, the capacity of Leishmania PSA protein to induce granzyme B production in humans with immunity to L. major and L. infantum infection. © 2014 Chamakh-Ayari et al.
Authors & Co-Authors
Chamakh-Ayari, Rym
Tunisia, Tunis
Institut Pasteur de Tunis
Bras-Gonçalves, Rachel
France, Montpellier
Ird Centre de Montpellier
Bahi-Jaber, Nargès
Tunisia, Tunis
Institut Pasteur de Tunis
France, Beauvais
Institut Polytechnique Lasalle Beauvais
Petitdidier, Elodie
France, Montpellier
Ird Centre de Montpellier
Markikou-Ouni, W.
Tunisia, Tunis
Institut Pasteur de Tunis
Aoun, Karim
Tunisia, Tunis
Institut Pasteur de Tunis
Moreno, Javier
Spain, Majadahonda
Centro Nacional de Microbiologia
Carrillo, E.
Spain, Majadahonda
Centro Nacional de Microbiologia
Salotra, Poonam
India, New Delhi
National Institute of Pathology
Kaushal, Himanshu
India, New Delhi
National Institute of Pathology
Negi, Narender Singh
India, New Delhi
National Institute of Pathology
Arevalo, Jorge Luis
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Falconi-Agapito, Francesca
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Privat, Angela
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Cruz, Maria
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Pagniez, Julie
France, Montpellier
Ird Centre de Montpellier
Papierok, Gérard Marie
France, Carros
Virbac sa
Rhouma, Faten Bel Haj
Tunisia, Tunis
Institut Pasteur de Tunis
Torres, Pilar
Spain, Madrid
Comunidad de Madrid
Lemesre, Jean Loup
France, Montpellier
Ird Centre de Montpellier
Chenik, Mehdi
Tunisia, Tunis
Institut Pasteur de Tunis
Meddeb-Garnaoui, Amel
Tunisia, Tunis
Institut Pasteur de Tunis
Statistics
Citations: 44
Authors: 22
Affiliations: 8
Identifiers
Doi:
10.1371/journal.pone.0092708
e-ISSN:
19326203