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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Sensitive detection of human papillomavirus in cervical, head/neck, and schistosomiasis-associated bladder malignancies
Proceedings of the National Academy of Sciences of the United States of America, Volume 102, No. 21, Year 2005
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Description
We assayed for the presence of human papilloma virus (HPV) DMA in serum and/or peripheral blood fraction (PBF) of individuals with cervical, head/neck, or bladder cancer due to schistosomiasis. Using mass spectroscopy coupled with competitive PCR, HPV DNA was detected at the individual molecule level by using "Mass-ARRAY" assays. The resultant sensitivity was superior to real-time fluorescent PCR-based assays, while specificity was maintained. Our principal findings were: (i) Virtually all tested cervical cancers and schistosomiasis-associated bladder cancers, and a plurality of head/neck cancers, are associated with HPV DNA in the tumor. (ii) All 27 bladder cancers due to schistosomiasis were associated with the presence of HPV-16 DNA, which can be detected in tumor and serum but not in PBF. In contrast, no serum HPV-16 DNA signal was detected in seven individuals with schistosomiasis-associated bladder cancers after surgical removal of the tumor. (iii) Among the head/neck cancers we studied, anterior tumors were more often associated with HPV DNA in tumor, serum, and/or PBF than posterior tumors. (iv) In cervical cancer, where all tumors contain HPV DNA, viral DNA could be detected often in serum and/or PBF. Further, HPV-16 DNA was detected in serum and/or PBF of most patients with untreated high-grade cervical dysplasia but disappeared if the dysplasia was eliminated. The sensitive, specific, and quantitative MassARRAY technique should make it feasible to monitor cancer occurrence and treatment and recurrence of malignancies and dysplasias associated with HPV DNA. © 2005 by The National Academy of Sciences of the USA.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC1140403/bin/pnas_102_21_7683__index.html
Authors & Co-Authors
Yang, H.
United States, Ann Arbor
University of Michigan, Ann Arbor
Yang, K.
United States, Ann Arbor
University of Michigan, Ann Arbor
Khafagi, A.
United States, Ann Arbor
University of Michigan, Ann Arbor
Tang, Y.
United States, Ann Arbor
University of Michigan, Ann Arbor
Carey, Thomas E.
United States, Ann Arbor
University of Michigan, Ann Arbor
Opipari, A. W.
United States, Ann Arbor
University of Michigan, Ann Arbor
Lieberman, Richard W.
United States, Ann Arbor
University of Michigan, Ann Arbor
Oeth, P. A.
United States, San Diego
Sequenom Inc.
Lancaster, Wayne D.
United States, Detroit
Wayne State University School of Medicine
Klinger, H. P.
United States, New York
Albert Einstein College of Medicine of Yeshiva University
Kaseb, Ahmed Omar
United States, Detroit
Henry Ford Hospital
Metwally, Ayman M.
Egypt, Giza
National Cancer Institute
Khaled, Hussein Mostafa
Egypt, Giza
National Cancer Institute
Kurnit, D. M.
United States, Ann Arbor
University of Michigan, Ann Arbor
Statistics
Citations: 50
Authors: 14
Affiliations: 6
Identifiers
Doi:
10.1073/pnas.0406904102
ISSN:
00278424
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Approach
Quantitative