Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch

One isoform of the branching enzyme (BE; EC 2.4.1.18) of potato (Solarium tuberosum L.) is known and catalyses the formation of α-1,6 bonds in a glucan chain, resulting in the branched starch component amylopectin. Constructs containing the antisense- or sense-orientated distal 1.5-kb part of a cDNA for potato BE were used to transform the amylose-free (amf) mutant of potato, the starch of which stains red with iodine. The expression of the endogenous BE gene was inhibited either largely or fully as judged by the decrease or absence of the Bb mRNA and protein. This resulted in a low percentage of starch granules with a small blue core and large red outer layer There was no effect on the amylose content, degree of branching or λmax of the iodine-stained starch. However when the physico-chemical properties of the different starch suspensions were assessed, differences were observed which although small indicated that starch in the transformants was different from that of the amf mutant.