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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus
Acta Tropica, Volume 99, No. 1, Year 2006
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Description
In order to investigate the transmission dynamics of Theileria parva (T. parva) by the brown ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions, detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay. A calf inoculated with a T. parva mixture became PCR-positive for T. parva infection on day 8 post-inoculation, and subsequently, nymphal ticks were introduced and maintained to feed on the infected calf for 6 days. Engorged nymphs were collected daily and allowed to molt into adults, and overall, 70.8% (121/171) of the adult ticks acquired the T. parva infection. Furthermore, the T. parva infection rate in ticks under field conditions was monitored by real-time PCR in R. appendiculatus ticks collected from a traditionally managed pastoral land of Zambia, on which Sanga breed cattle are traditionally reared and the area has endemic East Coast fever (ECF). A total of 70 cattle were randomly selected in the same area and 67 (95.7%) were found to be serologically positive for R. appendiculatus tick antigen (RIM36). Twenty-nine (43.3%) of the 67 serologically positive cattle were real-time PCR-positive for T. parva, although no piroplasms could be detected in the blood smears. Unexpectedly, out of 614 R. appendiculatus nymphal and adult ticks collected by flagging vegetation, 4.1% were positive for T. parva DNA. However, since the rate of transmission of T. parva from infected cattle to ticks and vice versa and the serological evidence of exposure to R. appendiculatus ticks in naturally exposed cattle were relatively high, it would be wise in such a case to consider vector control as well as vaccination against ECF as control measures. © 2006 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Konnai, Satoru
Japan, Sapporo
Graduate School of Veterinary Medicine
Imamura, Saiki
Japan, Sapporo
Graduate School of Veterinary Medicine
Nakajima, Chie
Japan, Sapporo
Graduate School of Veterinary Medicine
Witola, William Harold
Japan, Sapporo
Graduate School of Veterinary Medicine
Yamada, Shinji
Japan, Sapporo
Graduate School of Veterinary Medicine
Simuunza, Martin Chitolongo
Zambia, Lusaka
School of Veterinary Medicine
Nambota, Andrew Mubila
Zambia, Lusaka
School of Veterinary Medicine
Yasuda, Jun
Japan, Morioka
Iwate University
Ohashi, Kazuhiko
Japan, Sapporo
Graduate School of Veterinary Medicine
Onuma, Misao
Japan, Sapporo
Graduate School of Veterinary Medicine
Statistics
Citations: 43
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1016/j.actatropica.2006.06.008
ISSN:
0001706X
Research Areas
Genetics And Genomics
Study Approach
Quantitative
Study Locations
Zambia