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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine
Histochemistry and Cell Biology, Volume 125, No. 3, Year 2006
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Description
In vitro studies on the pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. Since many bacterial infections are swine-specific, studies on pathogenic mechanisms require appropriate cell lines of porcine origin. We have characterized the permanent porcine intestinal epithelial cell line, IPEC-J2, using a variety of methods in order to assess the usefulness of this cell line as an in vitro infection model. Electron microscopic analyses and histochemical staining revealed the cells to be enterocyte-like with microvilli, tight junctions and glycocalyx-bound mucin. The functional integrity of monolayers was determined by transepithelial electrical resistance (TEER) measurements. Both commensal bacteria and important bacterial pathogens were chosen for study based on their principally different infection mechanisms: obligate extracellular Escherichia coli, facultative intracellular Salmonella and obligate intracellular Chlamydia. We determined the colonization and proliferation of the bacteria on and within the host cells and monitored the host cell response. We verified the expression of mRNAs encoding the cytokines IL-1α, -6, -7, -8, -18, TNF-α and GM-CSF, but not TGF-β or MCP-1. IL-8 protein expression was enhanced by Salmonella invasion. We conclude that the IPEC-J2 cell line provides a relevant in vitro model system for porcine intestinal pathogen-host cell interactions. © Springer-Verlag 2005.
Authors & Co-Authors
Schierack, Peter
Germany, Hannover
Institut Für Mikrobiologie Und Tierseuchen
Nordhoff, Marcel
Germany, Hannover
Institut Für Mikrobiologie Und Tierseuchen
Weyrauch, Karl Dietrich
Germany, Berlin
Freie Universität Berlin
Amasheh, Salah
Germany, Berlin
Charité – Universitätsmedizin Berlin
Jores, Joerg
Germany, Hannover
Institut Für Mikrobiologie Und Tierseuchen
Kleta, Sylvia
Germany, Hannover
Institut Für Mikrobiologie Und Tierseuchen
Blikslager, Anthony Thomas
United States, Raleigh
Nc State University
Wieler, Lothar H.
Germany, Hannover
Institut Für Mikrobiologie Und Tierseuchen
Statistics
Citations: 297
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1007/s00418-005-0067-z
ISSN:
09486143
Research Areas
Infectious Diseases