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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds
PeerJ, Volume 2016, No. 2, Article e1704, Year 2016
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Description
Marine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mg/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTHtreated HepG2 cells presented also pro-apoptotic features, such as increased Annexin V/propidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Demethoxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cytotoxicity towards HepG2 cells. © 2016 Vizetto-Duarte et al.
Authors & Co-Authors
Vizetto-Duarte, Catarina
Portugal, Faro
Universidade do Algarve
Custódio, Luísa Margarida Batista
Portugal, Faro
Universidade do Algarve
Acosta, Gerardo A.
Spain, Barcelona
Irb Barcelona - Institute for Research in Biomedicine
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Lago, J. H.G.
Brazil, Sao Paulo
Universidade Federal de São Paulo
de Sousa, Carolina Bruno
Portugal, Faro
Universidade do Algarve
Rodrigues, Maria João
Portugal, Faro
Universidade do Algarve
Pereira, Hugo G.C.
Portugal, Faro
Universidade do Algarve
Barreira, Luísa A.
Portugal, Faro
Universidade do Algarve
Pilar Rauter, Amélia Pilar
Portugal, Lisbon
Faculdade de Ciências, Universidade de Lisboa
Alberício, Fernando
Spain, Barcelona
Irb Barcelona - Institute for Research in Biomedicine
Spain, Madrid
Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina
Spain, Barcelona
Universitat de Barcelona
Varela, J. Carlos Serafim
Portugal, Faro
Universidade do Algarve
Statistics
Citations: 34
Authors: 11
Affiliations: 8
Identifiers
Doi:
10.7717/peerj.1704
ISSN:
21678359
Research Areas
Cancer