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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Molecular tools separate harmful algal bloom species, Karenia mikimotoi, from different geographical regions into distinct sub-groups
Harmful Algae, Volume 10, No. 6, Year 2011
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Description
Karenia mikimotoi is a toxic dinoflagellate that is known to form extensive populations in the Eastern North Atlantic and Pacific Oceans that that impacts significantly on recreation and fisheries industries in these areas. Attempts to resolve the complex taxonomy within this " species" have been hampered by the requirement for fine-scale morphological analyses and by the lack of suitable genetic markers. Here we report the use of a novel combination of primer sets designed to facilitate amplification of the rDNA LSU and ITS, and the rbcL genes that can be used to discriminate between K. mikimotoi isolates originating from different geographical regions. We show that isolates from Europe and New Zealand are more closely related to each other than either is to isolates from Japan. Specific PCR-based primers were designed to amplify a region of the rbcL gene for subsequent high resolution analysis of the PCR amplicon melting temperatures. This innovative technique allows us to rapidly discriminate K. mikimotoi from distinct geographic localities and we propose the separation of K. mikimotoi into two distinct sub-species. © 2011 Elsevier B.V.
Authors & Co-Authors
Al-Kandari, Manal A.
United Kingdom, Plymouth
Marine Biological Association
Kuwait, Shuwaikh
Kuwait Institute for Scientific Research
United Kingdom, Plymouth
University of Plymouth
Highfield, Andrea C.
United Kingdom, Plymouth
Marine Biological Association
Hall, Matthew
United Kingdom, Plymouth
Marine Biological Association
Hayes, Paul
United Kingdom, Portsmouth
University of Portsmouth
Schroeder, Declan C.
United Kingdom, Plymouth
Marine Biological Association
Statistics
Citations: 31
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.hal.2011.04.017
ISSN:
15689883
Research Areas
Environmental
Genetics And Genomics