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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Role of Human Immunodeficiency Virus (HIV)-specific T-cell immunity in control of dual HIV-1 and HIV-2 infection
Journal of Virology, Volume 81, No. 17, Year 2007
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Description
Progressive immune dysfunction and AIDS develop in most cases of human immunodeficiency virus type 1 (HIV-1) infection but in only 25 to 30% of persons with HIV-2 infection. However, the natural history and immunologic responses of individuals with dual HIV-1 and HIV-2 infection are largely undefined. Based on our previous findings, we hypothesized that among patients with dual infection the control of HIV-1 is associated with the ability to respond to HIV-2 Gag epitopes and to maintain HIV-specific CD4+ T-cell responses. To test this, we compared the HIV-specific ex vivo IFN-γ enzyme-linked immunospot (ELISPOT) assay responses of 19 dually infected individuals to those of persons infected with HIV-1 or HIV-2 only. Further, we assessed the functional profile of HIV Gag-specific CD4+ and CD8+ T cells from nine HIV dually infected patients by using a multicolor intracellular cytokine staining assay. As determined by ELISPOT assay, the magnitude and frequency of IFN-γ-secreting T-cell responses to gene products of HIV-1 were higher than those to gene products of HIV-2 (2.64 versus 1.53 log10 IFN-γ spot-forming cells/106 cells [90% versus 63%, respectively].) Further, HIV-1 Env-, Gag-, and Nef- and HIV-2 Gag-specific responses were common; HIV-2 Nef-specific responses were rare. HIV-specific CD4+ T helper responses were detected in nine of nine dually infected subjects, with the majority of these T cells producing gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) and, to a lesser extent, interleukin-2. The HIV-1 plasma viral load was inversely correlated with HIV-2 Gag-specific IFN-γ-/TNF-α-secreting CD4+ and HIV-2 Gag-specific IFN-γ-secreting CD8+ T cells. In conclusion, the T-cell memory responses associated with containment of single HIV-1 and HIV-2 infection play a similar significant role in the immune control of dual HIV-1 and HIV-2 infection. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Zheng, Natalie N.
United States, Seattle
University of Washington
McElrath, Margaret Juliana
United States, Seattle
University of Washington
United States, Seattle
Clinical Research Division
Sow, Papa Salif
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Hawes, Stephen Edward
United States, Seattle
University of Washington
Diallo-Agne, Habibatou
Senegal, Dakar
Université Cheikh Anta Diop de Dakar
Stern, Joshua E.
United States, Seattle
University of Washington
Li, Fusheng
United States, Seattle
Fred Hutchinson Cancer Research Center
Mesher, Andrew
United States, Seattle
University of Washington
Robinson, Akeliah D.
United States, Seattle
Clinical Research Division
Gottlieb., Geoffrey S.
United States, Seattle
University of Washington
Huang, Yunda
United States, Seattle
Fred Hutchinson Cancer Research Center
Kiviat, Nancy B.
United States, Seattle
University of Washington
United States, Seattle
Harborview Medical Center
Statistics
Citations: 17
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1128/JVI.00117-07
ISSN:
0022538X
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases