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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Insecticide resistance in the dengue vector aedes aegypti from martinique: Distribution, mechanisms and relations with environmental factors
PLoS ONE, Volume 7, No. 2, Article e30989, Year 2012
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Description
Dengue is an important mosquito borne viral disease in Martinique Island (French West Indies). The viruses responsible for dengue are transmitted by Aedes aegypti, an indoor day-biting mosquito. The most effective proven method for disease prevention has been by vector control by various chemical or biological means. Unfortunately insecticide resistance has already been observed on the Island and recently showed to significantly reduce the efficacy of vector control interventions. In this study, we investigated the distribution of resistance and the underlying mechanisms in nine Ae. aegypti populations. Statistical multifactorial approach was used to investigate the correlations between insecticide resistance levels, associated mechanisms and environmental factors characterizing the mosquito populations. Bioassays revealed high levels of resistance to temephos and deltamethrin and susceptibility to Bti in the 9 populations tested. Biochemical assays showed elevated detoxification enzyme activities of monooxygenases, carboxylesterases and glutathione S-tranferases in most of the populations. Molecular screening for common insecticide target-site mutations, revealed the presence of the "knock-down resistance" V1016I Kdr mutation at high frequency (>87%). Real time quantitative RT-PCR showed the potential involvement of several candidate detoxification genes in insecticide resistance. Principal Component Analysis (PCA) performed with variables characterizing Ae. aegypti from Martinique permitted to underline potential links existing between resistance distribution and other variables such as agriculture practices, vector control interventions and urbanization. Insecticide resistance is widespread but not homogeneously distributed across Martinique. The influence of environmental and operational factors on the evolution of the resistance and mechanisms are discussed. © 2012 Marcombe et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3283601/bin/pone.0030989.s001.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC3283601/bin/pone.0030989.s002.xls
Authors & Co-Authors
Marcombe, Sébastien
France, Montpellier
Ird Centre de Montpellier
Blanc-Mathieu, Romain
France, Saint Martin D'heres
Université Grenoble Alpes
Pocquet, Nicolas
France, Montpellier
Ird Centre de Montpellier
Riaz, Muhammad Asam
France, Saint Martin D'heres
Université Grenoble Alpes
Poupardin, Rodolphe
France, Saint Martin D'heres
Université Grenoble Alpes
Sélior, Serge
Benin, Cotonou
Centre de Recherche Entomologique de Cotonou
Darriet, Frédéric
France, Montpellier
Ird Centre de Montpellier
Reynaud, Stéphane
France, Saint Martin D'heres
Université Grenoble Alpes
Yébakima, André
France, Martinique
Conseil Général de la Martinique
Corbel, Vincent
Benin, Cotonou
Centre de Recherche Entomologique de Cotonou
David, Jean Philippe
France, Saint Martin D'heres
Université Grenoble Alpes
Chandre, Fabrice
France, Montpellier
Ird Centre de Montpellier
Statistics
Citations: 221
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1371/journal.pone.0030989
e-ISSN:
19326203
Research Areas
Cancer
Infectious Diseases
Study Approach
Quantitative