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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Glycaemic control improves fibrin network characteristics in type 2 diabetes - A purified fibrinogen model
Thrombosis and Haemostasis, Volume 99, No. 4, Year 2008
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Description
Diabetic subjects have been shown to have altered fibrin network structures. One proposed mechanism for this is non-enzymatic glycation of fibrinogen due to high blood glucose. We investigated whether glycaemic control would result in altered fibrin network structures due to decreased fibrinogen glycation. Twenty uncontrolled type 2 diabetic subjects were treated with insulin in order to achieve glycaemic control. Twenty age- and body mass index (BMI)-matched non-diabetic subjects were included as a reference group. Purified fibrinogen, isolated from plasma samples was used for analysis. There was a significant decrease in fibrinogen glycation (6.81 to 5.02 mol glucose/mol fibrinogen) with a corresponding decrease in rate of lateral aggregation (5.86 to 4.62) and increased permeability (2.45 to 2.85 × 10-8 cm2) and lysis rate (3.08 to 3.27 μm/min) in the diabetic subjects after glycaemic control. These variables correlated with markers of glycaemic control. Fibrin clots of non-diabetic subjects had a significantly higher ratio of inelastic to elastic deformation than the diabetic subjects (0.10 vs. 0.09). Although there was no difference in median fiber diameter between diabetic and non-diabetic subjects, there was a small increase in the proportion of thicker fibers in the diabetic samples after glycaemic control. Results from SDS-PAGE indicated no detectable difference in factor XIIIa-crosslinking of fibrin clots between uncontrolled and controlled diabetic samples. Diabetic subjects may have altered fibrin network formation kinetics which contributes to decreased pore size and lysis rate of fibrin clots. Achievement of glycaemic control and decreased fibrinogen glycation level improves permeability and lysis rates in a purified fibrinogen model. © 2008 Schattauer GmbH.
Authors & Co-Authors
Pieters, Marlien
South Africa, Potchefstroom
North-west University
Covic, Namukolo Margaret
South Africa, Potchefstroom
North-west University
Van Der Westhuizen, F. H.
South Africa, Potchefstroom
North-west University
Nagaswami, Chandrasekaran
United States, Philadelphia
University of Pennsylvania
Baras, Yelena
United States, Philadelphia
University of Pennsylvania
Loots, Du Toit
South Africa, Potchefstroom
North-west University
Jerling, Johann C.
South Africa, Potchefstroom
North-west University
Elgar, Dale
South Africa, Potchefstroom
North-west University
Edmondson, Kathryn S.
United States, Philadelphia
University of Pennsylvania
van Zyl, Danie G.
South Africa, Pretoria
University of Pretoria
Rheeder, Paul
South Africa, Pretoria
University of Pretoria
Weisel, John W.
United States, Philadelphia
University of Pennsylvania
Statistics
Citations: 71
Authors: 12
Affiliations: 3
Identifiers
Doi:
10.1160/TH07-11-0699
ISSN:
03406245
Research Areas
Noncommunicable Diseases