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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
A novel expression system for production of soluble prion proteins in E. coli
Microbial Cell Factories, Volume 11, Article 6, Year 2012
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Description
Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, the need to obtain large quantities of the recombinant protein for research purposes has been essential. Currently, production of recombinant PrP is achieved by refolding protocols. Here, we show that the co-expression of two different PrP with the human Quiescin Sulfhydryl OXidase (QSOX), a human chaperone with thiol/disulfide oxidase activity, in the cytoplasm of E. coli produces soluble recombinant PrP. The structural integrity of the soluble PrP has been confirmed by nuclear magnetic resonance spectroscopy, demonstrating that properly folded PrP can be easily expressed in bacteria. Furthermore, the soluble recombinant PrP produced with this method can be used for functional and structural studies. © 2012 Abskharon et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Abskharon, Romany N.N.
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Ramboarina, Stephanie
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
El Hassan, Hassan
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Gad, Wael
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Brussels
Brussels Center for Redox Biology
Apostol, Marcin I.
United States, Cleveland
Case Western Reserve University
Giachin, Gabriele
Italy, Trieste
Scuola Internazionale Superiore Di Studi Avanzati
Legname, Giuseppe
Italy, Trieste
Scuola Internazionale Superiore Di Studi Avanzati
Italy, Genoa
Istituto Italiano Di Tecnologia
Italy, Basovizza
Elettra-sincrotrone Trieste S.c.p.a.
Steyaert, Jan
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Messens, Joris
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Belgium, Brussels
Brussels Center for Redox Biology
Soror, Sameh Hamdy
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Egypt, Helwan
Faculty of Pharmacy
Wohlkönig, Alexandre
Belgium, Ghent
Vlaams Instituut Voor Biotechnologie
Belgium, Brussels
Vrije Universiteit Brussel
Statistics
Citations: 29
Authors: 11
Affiliations: 8
Identifiers
Doi:
10.1186/1475-2859-11-6
e-ISSN:
14752859
Research Areas
Cancer