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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties
Food Chemistry, Volume 217, Year 2017
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Description
In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins. © 2016
Authors & Co-Authors
Khan, Faez Iqbal
China, Zhengzhou
Henan University of Technology
Yang, Bo
China, Guangzhou
South China University of Technology
Wang, Yonghua
China, Guangzhou
South China University of Technology
Statistics
Citations: 88
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1016/j.foodchem.2016.09.012
ISSN:
03088146
Research Areas
Cancer