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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Establishment and characterization of immortalized human amniotic epithelial cells
Cellular Reprogramming, Volume 15, No. 1, Year 2013
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Description
Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also have several characteristics similar to stem cells, and they are discarded after parturition. Thus, they could potentially be used in cell therapy with fewer ethical problems. HAEs have a short life, so our aim is to establish and characterize immortalized human amniotic epithelial cells (iHAEs). HAEs were introduced with viral oncogenes E6/E7 and with human telomerase reverse transcriptase (hTERT) to create iHAEs. These iHAEs have proliferated around 200 population doublings (PDs) for at least 12 months. High expression of stem cell markers (Oct 3/4, Nanog, Sox2, Klf4) and epithelial markers (CK5, CK18) were detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). These iHAEs were expanded in ultra-low-attachment dishes to form spheroids similarly to epithelial stem/precursor cells. High expression of mesenchymal (CD44, CD73, CD90, CD105) and somatic (CD24, CD29, CD271, Nestin) stem cell markers was detected by flow cytometry. The iHAEs showed adipogenic, osteogenic, neuronal, and cardiac differentiation abilities. In conclusion, the immortalization of HAEs with the characteristics of stem cells has been established, allowing these iHAEs to become useful for cell therapy and regenerative medicine. © 2013, Mary Ann Liebert, Inc.
Authors & Co-Authors
Zhou, Kaixuan
Japan, Toyama
University of Toyama
Koike, Chika
Japan, Toyama
University of Toyama
Yoshida, Toshiko
Japan, Toyama
University of Toyama
Okabe, Motonori
Japan, Toyama
University of Toyama
Fathy, Moustafa
Japan, Toyama
University of Toyama
Egypt, Minya
Faculty of Pharmacy
Kyo, Satoru
Japan, Kanazawa
Kanazawa University
Kiyono, Tohru
Japan, Chuo-ku
National Cancer Center Research Institute
Saito, Shigeru
Japan, Toyama
University of Toyama
Nikaido, Toshio
Japan, Toyama
University of Toyama
Statistics
Citations: 35
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.1089/cell.2012.0021
ISSN:
21524971
e-ISSN:
21524998
Research Areas
Cancer
Health System And Policy
Noncommunicable Diseases
Study Design
Cross Sectional Study