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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
DNA fragmentation, apoptosis and cell cycle arrest induced by zearalenone in cultured DOK, Vero and Caco-2 cells: Prevention by Vitamin E
Toxicology, Volume 192, No. 2-3, Year 2003
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Description
Zearalenone (ZEN) is a non-steroidal oestrogenic mycotoxin produced by several Fusarium species growing on cereals. ZEN and its metabolites bind to human oestrogen receptors and hence display oestrogenic and anabolic properties. Several lines of investigation suggest that ZEN may be genotoxic in vivo. ZEN damages DNA in Bacillus subtilis recombination tests, and it induces sister chromatid exchange and chromosomal aberration in CHO cells. ZEN also induces DNA-adduct formation in mouse tissues and SOS repair process in lysogenic bacteria. In the present study, ZEN genotoxicity has been confirmed in three cell-lines, Vero, Caco-2 and DOK at concentrations of 10, 20 and 40μM. Under these conditions, ZEN induces concentration-dependent DNA fragmentation resulting in DNA laddering patterns on agarose gel electrophoresis. This observation is consistent with apoptosis, which was confirmed by observations of formation of apoptotic bodies. Moreover, ZEN induces cell cycle arrest in the three cell-lines characterised by an increase of the number of cells in the G2/M phase of the cell cycle. Vitamin E (25μM) added simultaneously with ZEN partially reduces DNA fragmentation and apoptotic body formation after 24h incubation. Vitamin E may act by maintaining prolonged cell cycle arrest during which time DNA repair takes place. © 2003 Elsevier Ireland Ltd. All rights reserved.
Authors & Co-Authors
Abid-Essefi, Salwa
Tunisia, Monastir
Faculté de Médecine Dentaire de Monastir
Baudrimont, Isabelle
France, Bordeaux
Université de Bordeaux
Hassen, Wafa
Tunisia, Monastir
Faculté de Médecine Dentaire de Monastir
Ouanes, Zouhour
Tunisia, Monastir
Faculté de Médecine Dentaire de Monastir
Mobio, Théophile Amondo
France, Bordeaux
Université de Bordeaux
Anane, Rachid
France, Bordeaux
Université de Bordeaux
Creppy, Edmond Ekué
France, Bordeaux
Université de Bordeaux
Bacha, Hassen
Tunisia, Monastir
Faculté de Médecine Dentaire de Monastir
Statistics
Citations: 192
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1016/S0300-483X(03)00329-9
ISSN:
0300483X
Research Areas
Cancer
Genetics And Genomics