Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Development of gene expression markers of acute heat-light stress in reef-building Corals of the genus porites
PLoS ONE, Volume 6, No. 10, Article e26914, Year 2011
Notification
URL copied to clipboard!
Description
Coral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practices by making it possible to assess the distribution of bleaching risks among individual reef sites. Gene expression analysis based on quantitative PCR (qPCR) can be used as a diagnostic tool to determine coral condition in situ. We evaluated the expression of 13 candidate genes during heat-light stress in a common Caribbean coral Porites astreoides, and observed strong and consistent changes in gene expression in two independent experiments. Furthermore, we found that the apparent return to baseline expression levels during a recovery phase was rapid, despite visible signs of colony bleaching. We show that the response to acute heat-light stress in P. astreoides can be monitored by measuring the difference in expression of only two genes: Hsp16 and actin. We demonstrate that this assay discriminates between corals sampled from two field sites experiencing different temperatures. We also show that the assay is applicable to an Indo-Pacific congener, P. lobata, and therefore could potentially be used to diagnose acute heat-light stress on coral reefs worldwide. © 2011 Kenkel et al.
Authors & Co-Authors
Kenkel, C. D.
United States, Austin
The University of Texas at Austin
Aglyamova, Galina
United States, Austin
The University of Texas at Austin
Alamaru, Ada
Israel, Tel Aviv-yafo
Tel Aviv University
Bhagooli, Ranjeet
Mauritius, Reduit
University of Mauritius
Capper, Roxana
United States, Austin
The University of Texas at Austin
Cunning, Ross
United States, Miami
Rosenstiel School of Marine and Atmospheric Science
deVillers, Amanda
Guam, Mangilao
University of Guam Marine Laboratory
Haslun, Joshua A.
United States, Corpus Christi
Texas a and M University - Corpus Christi
Hédouin, Laetitia
France, Perpignan
Université de Perpignan Via Domitia
Keshavmurthy, Shashank
Taiwan, Taipei
Academia Sinica, Biodiversity Research Center
Kuehl, Kristin A.
United States, Miami
Florida International University
Mahmoud, Huda M.A.
Kuwait, Kuwait City
Kuwait University
Mcginty, E.
United States, Arlington
The University of Texas at Arlington
Montoya-Maya, Phanor H.
South Africa, Durban
Oceanographic Research Institute
Palmer, Caroline V.
Australia, Townsville
James Cook University
United Kingdom, Newcastle
Newcastle University
Pantile, Raffaella
Australia, Townsville
Australian Institute of Marine Science
Sánchez, Juan A.
Colombia, Bogota
Universidad de Los Andes, Colombia
Schils, Tom
Guam, Mangilao
University of Guam Marine Laboratory
Silverstein, Rachel N.
United States, Miami
Rosenstiel School of Marine and Atmospheric Science
Squiers, Logan B.
United States, Corpus Christi
Texas a and M University - Corpus Christi
Tang, Pei Ciao
United States, Lafayette
University of Louisiana at Lafayette
Goulet, T. L.
United States, University
University of Mississippi
Matz, M. V.
United States, Austin
The University of Texas at Austin
Statistics
Citations: 110
Authors: 23
Affiliations: 18
Identifiers
Doi:
10.1371/journal.pone.0026914
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Study Design
Cohort Study
Study Approach
Quantitative