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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy
Biosensors and Bioelectronics, Volume 21, No. 7, Year 2006
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Description
Rhodopsin, the G protein-coupled receptor (GPCR) which mediates the sense of vision, was prepared from calf eyes and used as receptor enriched membrane fraction. In this study it was immobilized onto gold electrode by two different techniques: Langmuir-Blodgett (LB) and a strategy based on a self-assembled multilayer. We demonstrated that Langmuir and LB films of rhodopsin are not stable. Thus, in this study a new protein multilayer was prepared on gold electrode by building up layer-by-layer a self-assembled multilayer. It is composed of a mixed self-assembled monolayer formed by MHDA and biotinyl-PE, followed by a biotin-avidin system which allows binding of biotinylated antibody specific to rhodopsin. The immobilization of rhodopsin in membrane fraction, by the specific antibody bound previously on self-assembled multilayer, was monitored with electrochemical impedance spectroscopy (EIS). In addition, the specificity and sensitivity of this self-assembled multilayer system to the presence of rhodopsin were investigated. No effect was observed when the system was in contact with olfactory receptor I7 in membrane fraction used for control measurements. All these results demonstrate that rhodopsin can be immobilized efficiently, specifically, quantitatively and stably on gold electrode through the self-assembled multilayer. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hou, Yanxia
France, Ecully
École Centrale de Lyon
China, Wuhan
Central China Normal University
Helali, Saloua
France, Ecully
École Centrale de Lyon
Tunisia, La Marsa
University of Carthage, Institut Préparatoire Aux Études Scientifiques et Techniques
Zhang, Aidong
China, Wuhan
Central China Normal University
Jaffrézic-Renault, Nicole J.
France, Ecully
École Centrale de Lyon
Martelet, Claudes
France, Ecully
École Centrale de Lyon
Vidić, Jasmina Minic
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Gorojankina, Tatiana
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Persuy, Marie Annick
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Pajot-Augy, Édith
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Salesse, Roland
France, Jouy-en-josas
Centre de Recherche Inrae Île-de-france - Jouy-en-josas - Antony
Bessueille, François
Spain, Barcelona
Laboratory of Nanobioengineering
Samitier, Josep
Spain, Barcelona
Laboratory of Nanobioengineering
Errachid, Abdelhamid
Spain, Barcelona
Laboratory of Nanobioengineering
Akimov, Volodymyr I.
Italy, San Cesario Di Lecce
National Nanotechnology Laboratory, Lecce
Reggiani, Lino
Italy, San Cesario Di Lecce
National Nanotechnology Laboratory, Lecce
Pennetta, Cecilia
Italy, San Cesario Di Lecce
National Nanotechnology Laboratory, Lecce
Alfinito, Eleonora
Italy, San Cesario Di Lecce
National Nanotechnology Laboratory, Lecce
Statistics
Citations: 103
Authors: 17
Affiliations: 6
Identifiers
Doi:
10.1016/j.bios.2005.06.002
ISSN:
09565663