Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A grape berry (Vitis vinifera L.) cation/proton antiporter is associated with berry ripening
Plant and Cell Physiology, Volume 48, No. 6, Year 2007
Notification
URL copied to clipboard!
Description
We have cloned and characterized VvNHX1, a gene encoding a vacuolar cation/H+ antiporter from Vitis vinifera cv. Cabernet Sauvignon. VvNHX1 belongs to the vacuolar NHX protein family and showed high similarity to other known vacuolar antiporters. The expression of VvNHX1 partially complements the salt- and hygromycin-sensitive phenotypes of an ena1-4 nhx1 yeast strain. Immunoblots of vacuoles of yeast expressing a VvNHX1, together with the expression of a VvNHX1-GFP (green fluorescent protein) chimera demonstrated that VvNHX1 localized to the vacuoles. VvNHX1 displayed low affinity K +/H+ and Na+/H+ exchange activities (12.8 and 40.2 mM, respectively). The high levels of expression of VvNHX1 during the véraison and post-véraison stages would indicate that the increase in vacuolar K+ accumulation, mediated by VvNHX1, is needed for vacuolar expansion. This process, together with the rapid accumulation of reducing sugars, would drive water uptake to the berry and the concomitant berry size increase, typical of the post-véraison stage of growth. © The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
Authors & Co-Authors
Hanana, M.
Tunisia, Hamam Lif
University of Carthage, Institut National de Recherche Scientifique et Technique Tunis
United States, Davis
University of California, Davis
Cagnac, Olivier
United States, Davis
University of California, Davis
Yamaguchi, Toshio
United States, Davis
University of California, Davis
Hamdi, Saïd
France, Bordeaux
Université de Bordeaux
Ghorbel, Abdelwahed
Tunisia, Hamam Lif
University of Carthage, Institut National de Recherche Scientifique et Technique Tunis
Blumwald, Eduardo
United States, Davis
University of California, Davis
Statistics
Citations: 70
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1093/pcp/pcm048
ISSN:
00320781
e-ISSN:
14719053
Research Areas
Environmental
Genetics And Genomics