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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Approaches for application of sub and supercritical fluid extraction for quantification of orbifloxacin from plasma and milk: Application to disposition kinetics
Analytica Chimica Acta, Volume 631, No. 1, Year 2009
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Description
Since its extensive development in the early 1980s, SFE has attracted considerable attention as a sample-preparation procedure. However, other different sample preparation procedures, including precipitation, liquid- and/or solid-phase extraction in biological fluids, also remain in use. In this investigation, SFE was introduced to isolate and identify orbifloxacin from plasma and milk. Four parameters, including the temperature and the pressure of supercritical fluid, modifier ratios, and dynamic extraction time, were evaluated and optimized to obtain the best yield of the analyte from the biological fluids. Determinations of the orbifloxacin (OBFX) in the extracts were carried out using HPLC-FLD. The optimum conditions of the extraction process that yielded the maximum analyte extraction efficiencies were 150 °C vs. 60 °C, 250 kg cm-2, 30% vs. 35% methanol, and 40 min vs. 20 min, for plasma and milk, respectively. The linearity of the calibration curves as well as the instrument LODs/LOQs were evaluated. Good linearity (at least r2 ≥ 0.999) of the calibration curves was obtained over the range from 0.2 to 0.01 μg mL-1. The method showed a good recovery rate (74.2-127.73%) and precision (RSDs: 1.64-20%). The instrumental LOD and LOQ values were 0.004 μg mL-1 vs. 0.01 μg mL-1 or 0.006 μg mL-1 vs. 0.02 μg mL-1, for plasma and milk, respectively. The method was successfully applied to estimate the pharmacokinetic variables of orbifloxacin in lactating does. To the best of our knowledge, this is the first time that SFE has been applied to isolate an antimicrobial agent from biological fluids. This method is promising for clinical applications and for pharmacokinetic studies of various pharmaceuticals in biological fluids. © 2008 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Abd El-Aty, A. M.
South Korea, Seoul
Konkuk University
Egypt, Giza
Faculty of Veterinary Medicine
Choi, Jeong-Heui
South Korea, Gwangju
College of Agriculture and Life Sciences
Ko, Ma Wou
South Korea, Gwangju
College of Agriculture and Life Sciences
Khay, Sathya
South Korea, Gwangju
College of Agriculture and Life Sciences
Goudah, Ayman M.
Egypt, Giza
Faculty of Veterinary Medicine
Shin, Ho-chul
South Korea, Seoul
Konkuk University
Kim, Jin-suk
South Korea, Seoul
Konkuk University
Chang, Byungjoon
South Korea, Seoul
Konkuk University
Lee, Chiho
South Korea, Seoul
Konkuk University
Shim, Jae-Han
South Korea, Gwangju
College of Agriculture and Life Sciences
Statistics
Citations: 18
Authors: 10
Affiliations: 3
Identifiers
Doi:
10.1016/j.aca.2008.10.023
e-ISSN:
18734324