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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Development of a high performance liquid chromatographic method for systematic quantitative analysis of chemical constituents in rhubarb
Chemical and Pharmaceutical Bulletin, Volume 54, No. 7, Year 2006
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Description
HPLC methods for the systematic determination of 30 compounds in Rhei Rhizoma (rhubarb) were developed. Using a combination of mobile phase gradient conditions and UV detection at 280 nm, all 30 compounds were separated satisfactorily with low detection limits (0.05-2 μg/ml). The developed methods provided a reliable calibration curve for each compound. By adopting these methods, the determination of 30 compounds in three kinds of rhubarb samples, derived from Rheum tanguticum, R. palmatum and R. officinale, was achieved. The constituent pattern of each rhubarb was clearly characterized through the quantitative composition of 30 major constituents of rhubarb. © 2006 Pharmaceutical Society of Japan.
Authors & Co-Authors
Komatsu, Katsuko
Japan, Toyama
University of Toyama
Nagayama, Yorinobu
Japan, Toyama
University of Toyama
Tanaka, Ken
Japan, Toyama
University of Toyama
Ling, Yun
China, Beijing
Beijing Yanjing Hospital
Basnet, Purusotam
Nepal, Pokhara
Pokhara University
Meselhy, M. R.
Egypt, Cairo
Faculty of Pharmacy
Statistics
Citations: 50
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1248/cpb.54.941
ISSN:
00092363
e-ISSN:
13475223
Study Approach
Quantitative