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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Pentose phosphate metabolism in Leishmania mexicana
Molecular and Biochemical Parasitology, Volume 130, No. 2, Year 2003
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Description
The metabolism of pentose phosphates was studied in Leishmania mexicana promastigotes. Each of the enzymes of the classical pentose phosphate pathway (PPP) has been identified and specific activities measured. Functioning of the PPP was demonstrated in non-growing cells by measuring the evolution of 14CO2 from [1-14C]D-glucose and [6- 14C]D-glucose under normal conditions and also under selective stimulation of the PPP by exposure to methylene blue. The proportion of glucose which passes through the PPP increases in the latter condition, thus suggesting a protective role against oxidant stress. The incorporation into nucleic acids of ribose 5-phosphate provided via either glucose or free ribose was also determined. Results indicate that the PPP enables glucose to serve as a source of ribose 5-phosphate in nucleotide biosynthesis. Moreover, free ribose is incorporated efficiently, implying the presence of a ribose uptake system and also of ribokinase. Ribose was shown to be accumulated by a carrier mediated process in L. mexicana promastigotes and ribokinase activity was also measured in these cells. © 2003 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Maugeri, Dante Abel
Argentina, Chascomus
Instituto Tecnológico Chascomús
Cazzulo, Juan Jose
Argentina, Chascomus
Instituto Tecnológico Chascomús
Burchmore, Richard J.
United Kingdom, Glasgow
University of Glasgow
Barrett, Michael Peter
United Kingdom, Glasgow
University of Glasgow
Ogbunude, Patrick O.J.
Nigeria, Naukka
University of Nigeria
Statistics
Citations: 81
Authors: 5
Affiliations: 3
Identifiers
Doi:
10.1016/S0166-6851(03)00173-7
ISSN:
01666851