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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Induction of HT-29cells apoptosis by lactobacilli isolated from fermented products
Research in Microbiology, Volume 165, No. 3, Year 2014
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Description
A total of 138 lactobacillus strains were obtained from the traditional fermented foods of minority nationalities or infant faeces, respectively. The lactobacillus strains were screened for anticancer effects and probiotic potential. The results showed that 10 strains exerted anti-proliferative activity and higher adhering capability on HT-29cells. They were then screened for resistance to biological barriers (acid and bile salts), and the four most promising strains were selected. Further analysis revealed that the 4 strains (cell walls and cytoplasm extracts) displayed the high anti-proliferative activity and the large extent of DNA strand breakage in individual cells. Through the selected procedure, cell walls that were extracted from X12, M5 and K14 strains induced apoptosis in HT-29cells. Further investigation confirmed that apoptosis-inducing ability of cell wall extracts was attributed to the breakdown of mitochondrial membrane potential, which is a known initiation of apoptotic mitochondrial pathway. Cell walls from X12, M5 and K14 strains, were determined to be less harmful to noncancerous Vero cells than to human colon cancer HT-29cells. These findings suggested that X12, M5 and K14 strains opposing the ability to induce HT-29cells apoptosis, and cell wall extracts were involved in this apoptosis induction. © 2014 Institut Pasteur.
Authors & Co-Authors
Wang, Shu Mei
China, Harbin
Harbin Institute of Technology
China, Harbin
Harbin University
Zhang, Lan Wei
China, Harbin
Harbin Institute of Technology
Fan, Rong Bo
China, Harbin
Harbin Institute of Technology
China, Qingdao
Qingdao Agricultural University
Han, Xue
China, Harbin
Harbin Institute of Technology
Yi, Hua Xi
China, Harbin
Harbin Institute of Technology
Zhang, Li Li
China, Harbin
Harbin Institute of Technology
Xue, Chao Hui
China, Harbin
Harbin Institute of Technology
Li, Hong Bo
China, Harbin
Harbin Institute of Technology
Zhang, Yan He
China, Harbin
Harbin Institute of Technology
Shigwedha, Nditange
China, Harbin
Harbin Institute of Technology
Namibia, Windhoek
University of Namibia
Statistics
Citations: 63
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.resmic.2014.02.004
ISSN:
09232508
e-ISSN:
17697123
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health