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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Partially purified polygalacturonase from Aspergillus niger (SA6)
African Journal of Biotechnology, Volume 9, No. 52, Year 2010
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Description
Polygalacturonase (PG) was isolated from Aspergillus niger (A. niger) (SA6), partially purified and characterized. The PG showed two bands on SDS-PAGE suggesting an "endo and exo PG with apparent molecular weights of 35 and 40 KDa, respectively. It was purified 9-fold with a yield of 0.18% and specific activity of 246 μmole/ml/mg. The KM and Vmax of the enzyme were 2.74 mg/ml and 0.78 μmole/min/mg, respectively. The optimum temperature and optimum pH of the enzyme were 40°C and 4.5, respectively. The PG was found to be more stable to temperature changes than to pH changes. © 2010 Academic Journals.
Authors & Co-Authors
Lawal, Buga Mohammed
Nigeria, Abuja
Raw Materials Research and Development Council
Ibrahim, Sani K.
Nigeria, Zaria
Ahmadu Bello University
Nok, Andrew Jonathan
Nigeria, Zaria
Ahmadu Bello University
Statistics
Citations: 3
Authors: 3
Affiliations: 2
Identifiers
ISSN:
16845315
Study Locations
Niger