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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Strain differentiation of Mycobacterium tuberculosis complex isolated from sputum of pulmonary tuberculosis patients
International Journal of Infectious Diseases, Volume 13, No. 2, Year 2009
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Description
Objective: This study represents an early attempt to determine the diversity of Mycobacterium tuberculosis in Egypt, particularly of drug-resistant strains. Methods: We characterized 45 Mycobacterium tuberculosis complex isolates from sputum samples of Egyptian patients with pulmonary tuberculosis, in order to establish a database of strain types and antimicrobial susceptibility patterns. Results: One Mycobacterium bovis and 44 Mycobacterium tuberculosis (MTB) isolates were identified by PCR-restriction fragment length polymorphism (RFLP) analysis of the oxyR gene. Twenty-five (56.8%) of the 44 MTB isolates were susceptible in vitro to all anti-tuberculosis drugs tested; five (11.4%) were mono-resistant to isoniazid or streptomycin (four were resistant to streptomycin and only one was resistant to isoniazid) and 14 (31.8%) were resistant to more than one drug (multidrug-resistant, MDR). Among the 44 MTB isolates tested by RFLP analysis in this study, 40 different RFLP patterns were obtained. The number of IS6110 copies ranged from 5 to 16. Studying the IS6110 RFLP patterns indicated that the 44 isolates did not cluster together but were generally scattered. None of the 14 MDR isolates were clustered. Twenty-two different spoligotypes were identified among the 44 MTB isolates, of which 13 were unique. The remaining 31 isolates were grouped into nine clusters of strains sharing identical spoligotypes. Conclusions: We have demonstrated evidence of diversity among the drug-susceptible and resistant MTB strains. Continued surveillance for strains of MTB involved in pulmonary tuberculosis in Egypt, and especially for drug-resistant strains, is warranted. © 2008 International Society for Infectious Diseases.
Authors & Co-Authors
Abbadi, Said Hamed
Egypt, Ismailia
Faculty of Medicine
El-Hadidy, Gehan S.
Egypt, Ismailia
Faculty of Medicine
Gomaa, Nahed I.M.
Egypt, Ismailia
Faculty of Medicine
Cooksey, R. C.
United States, Atlanta
Std
Statistics
Citations: 32
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijid.2008.06.020
ISSN:
12019712
Research Areas
Genetics And Genomics
Study Locations
Egypt