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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Promoter hypermethylation of CDH13, DAPK1 and TWIST1 genes in precancerous and cancerous lesions of the uterine cervix
Pathology Research and Practice, Volume 207, No. 1, Year 2011
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Description
Aberrant DNA methylation is an early event in carcinogenesis and could serve as an additional molecular marker for the early diagnosis. The study was performed to investigate the promoter methylation of DAPK1, CDH13, and TWIST1 genes in uterine cervix lesions in an effort to examine whether this epigenetic event is involved in the process of cervical carcinogenesis, and whether it might be used as a molecular marker of cervical lesions.We conducted a retrospective study of 60 uterine cervix specimens, including 8 normal tissue samples, 10 benign lesions, 28 precancerous lesions (CIN1-3), and 14 squamous cell carcinomas (SCC). DNA hypermethylation was investigated using methylation-specific PCR. Immunohistochemistry was used to find p16INK4A overexpression.No hypermethylated promoters were detected in normal tissues and benign lesions. However, promoter hypermethylation of CDH13, TWIST1, and DAPK1 increased progressively from CIN1 to cancer, reaching values higher than 50% for cancer. DAPK1 and CDH13 displayed a significantly increased frequency of promoter methylation with progressively more severe cervical neoplasia (p<0.05). A statistically significant association was observed between p16INK4A expression and hypermethylation of DAPK1, TWIST1, and CDH13 (p<0.0001).Hypermethylation of CDH13, DAPK1, and TWIST1 promoters is an early event in the initiation and progression of cervix neoplasia. CDH13, DAPK1, and TWIST1 genes are potential biomarkers of cervical cancer risk. © 2010 Elsevier GmbH.
Authors & Co-Authors
Missaoui, Nabiha
Tunisia, Sousse
Faculty of Medicine
France, Lyon
Le Centre Régional de Lutte Contre le Cancer Léon Bérard
Tunisia, Sousse
Hopital Farhat Hached Sousse
Hmissa, Sihem
Tunisia, Sousse
Faculty of Medicine
Tunisia, Sousse
Hopital Farhat Hached Sousse
Trabelsi, Amel
Tunisia, Sousse
Hopital Farhat Hached Sousse
Traoré, Cheick Bougadari
France, Lyon
Hopital Edouard Herriot
Mokni, Moncef
Tunisia, Sousse
Hopital Farhat Hached Sousse
Dante, Robert
France, Lyon
Le Centre Régional de Lutte Contre le Cancer Léon Bérard
Frappart, Lucien
France, Lyon
Le Centre Régional de Lutte Contre le Cancer Léon Bérard
France, Lyon
Hopital Edouard Herriot
Statistics
Citations: 37
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1016/j.prp.2010.11.001
ISSN:
03440338
e-ISSN:
16180631
Research Areas
Cancer
Genetics And Genomics
Study Design
Cohort Study