Quantitative characterization and analysis of granule transformations: Role of intermittent gas sparging in a super high-rate anaerobic system

Knowledge of leveraging biomass characteristics is essential for achieving a microbial community with a desired structure to optimize anaerobic bioreactor performance. This study investigates the successive granule transformations in a high-rate anaerobic system with intermittent gas sparging and sequential increases in organic loading rates (OLRs), by establishing the correlations between the granule microstructures and reactor operating parameters. Over the course of a 196-day lab-scale trial, the granules were visualized in various stages using scanning electron microscopy, and digital image processing was applied for further quantifying their surface properties. Correlation analyses revealed that irregularities of the granule microstructures (surface properties, specific surface area and pore volume) emerged at stage 4 when the OLR was 13.31 kg COD/m3·day and in stage 5 in the absence of gas sparging. The loading ratio (substrate surface loading to upward velocity) was identified to be the main parameter controlling the granule transformations, and the surface structures were classified into three categories for further interpretation. Confocal laser scanning microscopy analyses showed that the granule core started to hollow out from stage 4. It is also found that a rough granule surface helped accelerate the growth of the granular diameter under gas sparging. Overall, this study not only establish quantitative correlations between the granules microstructures and reactor operating parameters, but also shed light on the use of intermittent gas sparging to control the surface properties of anaerobic granules in high-rate anaerobic bioreactors.