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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates
PLoS ONE, Volume 9, No. 6, Article e98758, Year 2014
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Description
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+ 2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. © 2014 Dorneles et al.
Authors & Co-Authors
Dorneles, Elaine M.S.
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Santana, Jordana A.
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Ribeiro, Dayana
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Dorella, Fernanda Alves
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Guimarães, Alessandro S.
Brazil, Brasilia
Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Brazil, Lavras
Universidade Federal de Lavras
Moawad, Mohamed Salah
Egypt, Giza
Cairo University
Selim, Salah A.
Egypt, Giza
Cairo University
Garaldi, Ana Luiza M.
Brazil, Rio de Janeiro
Universidade do Estado do Rio de Janeiro, Faculdade de Ciencias Medicas
Miyoshi, Anderson
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Ribeiro, Márcio G.
Brazil, Sao Paulo
Universidade Estadual Paulista Júlio de Mesquita Filho
Gouveia, Aurora M.G.
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Carvalho Azevedo, Vasco Ariston
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Heinemann, Marcos Bryan
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Lage, Andrey P.
Brazil, Belo Horizonte
Universidade Federal de Minas Gerais
Statistics
Citations: 14
Authors: 14
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0098758
e-ISSN:
19326203