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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Measure of viral load by using the Abbott real-time HIV-1 assay on dried blood and plasma spot specimens collected in 2 rural dispensaries in Cameroon
Journal of Acquired Immune Deficiency Syndromes, Volume 52, No. 1, Year 2009
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Description
BACKGROUND: This study aimed to evaluate the use of dried blood spots (DBSs) and dried plasma spots (DPSs) locally collected in 2 rural dispensaries in Cameroon for the quantification of HIV-1 RNA. METHODS: Forty-one subjects were sampled and spots of whole blood and plasma were deposited onto Whatman 903 cards and dried at ambient temperature under local conditions. Two sets of DBS and DPS cards were done per patient. The rest of the liquid plasma (LP) was frozen until use. LPs were tested at the "Chantal Biya" International Reference Centre (Yaoundé, Cameroon) by the Abbott Real-Time HIV-1 assay (Abbott Molecular Diagnostics, Wiesbaden, Germany). One series of DBS and DPS was transported and tested between 2 and 6 weeks later at the Virology Laboratory of Saint-Etienne (France). The second series was routed by mail and tested after up to 3 months of storage at ambient temperature. RESULTS: From the first series, the correlation rate between viral loads obtained from LP and DBS, and from LP and DPS, was 0.98 and 0.99, respectively; specificity of DBS and DPS results was 100%. The results obtained from the second series indicate a great stability of DBS after long-term storage. CONCLUSION: This study demonstrates that DBSs collected under local conditions in resource-limited settings are suitable for the differed quantification of HIV-1 RNA. © 2009 Lippincott Williams & Wilkins, Inc.
Authors & Co-Authors
Mbida, André Dieudonné
Cameroon, Yaounde
Inst. Rech. Med. D'etud. Plantes M.
Sosso, Samuel Martin
Cameroon, Yaounde
Chantai Biya International Center for Research and Prevention of Hiv/aids Circb
Flori, Pierre
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Saoudin, Henia
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Lawrence, Philip
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Monny-Lobé, Marcel
Cameroon, Yaounde
Chantai Biya International Center for Research and Prevention of Hiv/aids Circb
Oyono, Yves
Cameroon, Yaounde
Inst. Rech. Med. D'etud. Plantes M.
Ndzi, Edward Shafe
Cameroon, Yaounde
Inst. Rech. Med. D'etud. Plantes M.
Cappelli, Giulia
Cameroon, Yaounde
Chantai Biya International Center for Research and Prevention of Hiv/aids Circb
Lucht, Frederick R.
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Pozzetto, Bruno
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
Ouwe-Missi-Oukem-Boyer, Odile N.
Cameroon, Yaounde
Chantai Biya International Center for Research and Prevention of Hiv/aids Circb
Bourlet, Thomas
France, Saint-etienne
Centre Hospitalier Universitaire de Saint Etienne
France, Saint-etienne
Université Jean Monnet - Saint Etienne Faculté de Médecine Jacques Lisfranc
Statistics
Citations: 61
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.1097/QAI.0b013e3181aeccbc
ISSN:
15254135
Research Areas
Health System And Policy
Infectious Diseases
Study Locations
Cameroon