Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Candidatus Rickettsia hoogstraalii in Ethiopian Argas persicus ticks
Ticks and Tick-borne Diseases, Volume 3, No. 5-6, Year 2012
Notification
URL copied to clipboard!
Description
Ethiopian soft ticks Argas persicus, hard ticks including both Amblyomma variegatum and Rhipicephalus (Boophilus) spp., and fleas were collected from livestock, traditional human dwellings, and cracks and crevices of trees. They were assessed in pools for the presence of Rickettsia using PCR-based methods. The extracted tick DNA was subjected to molecular screening for Rickettsia, which revealed 50.5% of the pooled samples to be positive for Rickettsia spp. These were then subjected to multi-gene analysis using both outer surface proteins and housekeeping genes with proven discriminatory potential. Sequencing of the citrate synthase and outer membrane genes clearly led to the identification of three distinct rickettsial species, Candidatus Rickettsia hoogstraalii in Argas persicus ticks; R. africae in hard tick pools, and R. felis in fleas. Furthermore, we demonstrated the presence of the plasmid-borne small heat-shock protein gene hsp2 in DNA from A. persicus ticks suggesting that Candidatus R. hoogstraalii carried by these ticks possess a plasmid. Unlike chromosomal gene sequences, the hsp2 gene failed to cluster with Candidatus R. hoogstraalii, instead falling into an isolated separate clade, suggesting a different origin for the plasmid. © 2012 Elsevier GmbH.
Authors & Co-Authors
Pader, Vera
United Kingdom, London
University of East London
Buniak, Joanna Nikitorowicz
United Kingdom, London
University of East London
Abdissa, Alemseged L.
Ethiopia, Jimma
Jimma University
Adamu, Haileeysus
Ethiopia, Jimma
Jimma University
Tolosa, Tadele
Ethiopia, Jimma
Jimma University
Gashaw, Abebaw
Ethiopia, Jimma
Jimma University
Cutler, Ronald R.
United Kingdom, London
School of Biological and Chemical Sciences Queen Mary University of London
Cutler, Sally Jane
United Kingdom, London
University of East London
Statistics
Citations: 31
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.ttbdis.2012.10.021
ISSN:
1877959X
e-ISSN:
18779603
Research Areas
Genetics And Genomics