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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
OMIP-056: Evaluation of Human Conventional T Cells, Donor-Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining
Cytometry Part A, Volume 95, No. 7, Year 2019
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Description
A 26-color staining panel was developed to profile human antigen-specific T cells in an intracellular cytokine staining (ICS) assay using peptide pools to various antigens of interest. In addition to multiple functional markers, the panel includes differentiation/activation markers and markers to assess γδ, mucosal-associated invariant T, and NK T cells as well as conventional NK cells. Panel optimization was performed using previously cryopreserved PBMC from healthy adults, and then, expression of key functional markers in the panel was cross-validated against a validated ICS assay used in the HIV Vaccine Trials Network (HVTN). The panel is currently being used to evaluate the responses to tuberculosis and malaria vaccine candidates in volunteers from different geographic areas. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC6663658/bin/CYTO-95-722-s001.docx
Authors & Co-Authors
Dintwe, One B.
South Africa, Cape Town
Cape Town Hvtn Immunology Laboratory
United States, Seattle
Fred Hutchinson Cancer Research Center
Rohith, Shamiska
South Africa, Cape Town
Cape Town Hvtn Immunology Laboratory
Schwedhelm, Katharine V.
United States, Seattle
Fred Hutchinson Cancer Research Center
McElrath, Margaret Juliana
United States, Seattle
Fred Hutchinson Cancer Research Center
United States, Seattle
University of Washington
Andersen-Nissen, Erica
South Africa, Cape Town
Cape Town Hvtn Immunology Laboratory
United States, Seattle
Fred Hutchinson Cancer Research Center
de Rosa, Stephen C.
United States, Seattle
Fred Hutchinson Cancer Research Center
United States, Seattle
University of Washington
Statistics
Citations: 16
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1002/cyto.a.23753
ISSN:
15524922
Research Areas
Infectious Diseases