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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Acclimation of Saccharomyces cerevisiae to low temperature: A chemostat-based transcriptome analysis
Molecular Biology of the Cell, Volume 18, No. 12, Year 2007
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Description
Effects of suboptimal temperatures on transcriptional regulation in yeast have been extensively studied in batch cultures. To eliminate indirect effects of specific growth rates that are inherent to batch-cultivation studies, genome-wide transcriptional responses to low temperatures were analyzed in steady-state chemostats, grown at a fixed specific growth rate (0.03 h -1). Although in vivo metabolic fluxes were essentially the same in cultures grown at 12 and at 30°C, concentrations of the growth-limiting nutrients (glucose or ammonia) were higher at 12°C. This difference was reflected by transcript levels of genes that encode transporters for the growth-limiting nutrients. Several transcriptional responses to low temperature occurred under both nutrient-limitation regimes. Increased transcription of ribosome-biogenesis genes emphasized the importance of adapting protein-synthesis capacity to low temperature. In contrast to observations in cold-shock and batch-culture studies, transcript levels of environmental stress response genes were reduced at 12°C. Transcription of trehalose-biosynthesis genes and intracellular trehalose levels indicated that, in contrast to its role in cold-shock adaptation, trehalose is not involved in steady-state low-temperature adaptation. Comparison of the chemostat-based transcriptome data with literature data revealed large differences between transcriptional reprogramming during long-term low-temperature acclimation and the transcriptional responses to a rapid transition to low temperature. © 2007 by The American Society for Cell Biology.
Authors & Co-Authors
Tai, Siew L.
Netherlands, Delft
Delft University of Technology
Netherlands, Delft
Kluyver Centre for Genomics of Industrial Fermentation
South Africa, Stellenbosch
Stellenbosch University
Daran-Lapujade, Pascale A.S.
Netherlands, Delft
Delft University of Technology
Netherlands, Delft
Kluyver Centre for Genomics of Industrial Fermentation
Walsh, Michael C.
Netherlands, Zoeterwoude
Heineken Supply Chain
Pronk, Jack T.
Netherlands, Delft
Delft University of Technology
Netherlands, Delft
Kluyver Centre for Genomics of Industrial Fermentation
Daran, Jean Marc
Netherlands, Delft
Delft University of Technology
Netherlands, Delft
Kluyver Centre for Genomics of Industrial Fermentation
Statistics
Citations: 120
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1091/mbc.E07-02-0131
ISSN:
10591524
Research Areas
Noncommunicable Diseases