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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Microscopy and molecular biology for the diagnosis and evaluation of malaria in a hospital in a rural area of Ethiopia
Malaria Journal, Volume 11, Article 199, Year 2012
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Description
Background: Malaria is a leading public health problem in Ethiopia. Accurate diagnosis of Plasmodium infections is crucial for the reduction of malaria in tropical areas and for epidemiological studies. The role of light microscopy (LM) as gold standard has been questioned and, therefore, new molecular methods have been developed for the detection of Plasmodium species. The aim of the present work was to compare different malaria diagnostic methods in order to detect the most common species of Plasmodium and to broaden the knowledge of malaria prevalence in a hospital in a rural area in Ethiopia. Methods. A cross-sectional survey of 471 individuals was carried out in a hospital in the rural area of Gambo (Ethiopia). Blood samples were prepared for microscopic observation and collected in filter paper for Seminested-Multiplex PCR (SnM-PCR) and real time PCR (qPCR) testing. The SnM-PCR was considered as the gold standard technique and compared with the rest. Thus, agreement between SnM-PCR and LM was determined by calculating Kappa Statistics and correlation between LM and qPCR quantification was calculated by pair-wise correlation co-efficient. Results: Samples analysed by LM and SnM-PCR were positive for Plasmodium sp. 5.5% and 10.5%, respectively. Sensitivity was 52.2% by LM and 70% by qPCR. Correlation co-efficient between microscopy counts and qPCR densities for Plasmodium vivax was R2=0.586. Prevalence was estimated at 7% (95% CI: 4.7-9.3). Plasmodium vivax was the dominant species detected and the difference was statistically significant ( 2=5.121 p<0.05). The highest prevalence of the parasite (10.9%) was observed in age groups under 15years old. Conclusion: Accurate malaria diagnostic methods have a great effect in the reduction of the number of malaria-infected individuals. SnM-PCR detection of malaria parasites may be a very useful complement to microscopic examination in order to obtain the real prevalence of each Plasmodium species. Although SnM-PCR shows that it is a good tool for the determination of Plasmodium species, today light microscopy remains the only viabletool for malaria diagnosis in developing countries. Therefore, re-inforcement in the training of microscopists is essential for making the correct diagnosis of malaria. Plasmodium vivax was the predominant species in Gambo, a meso-endemic area for this species. © 2012 Santana-Morales et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Santana-Morales, María Ángeles
Spain, San Cristobal de la Laguna
Universidad de la Laguna
Afonso-Lehmann, Raquel N.
Spain, San Cristobal de la Laguna
Universidad de la Laguna
Quispe, Maria A.
Spain, San Cristobal de la Laguna
Universidad de la Laguna
Reyes, Francisco
Ethiopia, Gambo, West-arsi
Gambo General Rural Hospital
Berzosa, Pedro J.
Spain, Madrid
Centro Nacional de Medicina Tropical
Benito, Agustìn
Spain, Madrid
Centro Nacional de Medicina Tropical
Valladares, Basilio
Spain, San Cristobal de la Laguna
Universidad de la Laguna
Martínez-Carretero, Enrique
Spain, San Cristobal de la Laguna
Universidad de la Laguna
Statistics
Citations: 21
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1186/1475-2875-11-199
e-ISSN:
14752875
Research Areas
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Ethiopia