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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates
Journal of Clinical Microbiology, Volume 46, No. 10, Year 2008
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Description
Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine drug susceptibility testing of Mycobacterium tuberculosis for pyrazinamide is, in many laboratories, not performed. The objective of our study was to generate information on pyrazinamide susceptibility among South African MDR and susceptible M. tuberculosis isolates from pulmonary tuberculosis patients. Seventy-one MDR and 59 fully susceptible M. tuberculosis isolates collected during the national surveillance study (2001 to 2002, by the Medical Research Council, South Africa) were examined for pyrazinamide susceptibility by the radiometric Bactec 460 TB system, pyrazinamidase activity (by Wayne's assay), and sequencing of the pncA gene. The frequency of pyrazinamide resistance (by the Bactec system) among the MDR M. tuberculosis isolates was 37 of 71 (52.1%) and 6 of 59 (10.2%) among fully sensitive isolates. A total of 25 unique mutations in the pncA gene were detected. The majority of these were point mutations that resulted in amino acid substitutions. Twenty-eight isolates had identical mutations in the pncA gene, but could be differentiated from each other by a combination of the spoligotype patterns and 12 mycobacterial interspersed repetitive-unit loci. A high proportion of South African MDR M. tuberculosis isolates were resistant to pyrazinamide, suggesting an evaluation of its role in patients treated previously for tuberculosis as well as its role in the treatment of MDR-TB. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Mphahlele, Matsie
Norway, Bergen
Universitetet I Bergen
South Africa, Tygerberg
South African Medical Research Council
Syre, Heidi
Norway, Bergen
Universitetet I Bergen
Norway, Bergen
Haukeland Universitetssjukehus
Valvatne, Håvard
Norway, Bergen
Universitetet I Bergen
Stavrum, Ruth
Norway, Bergen
Universitetet I Bergen
Norway, Bergen
Haukeland Universitetssjukehus
Mannsåker, Turid
Norway, Oslo
Folkehelseinstituttet
Muthivhi, Tshilidzi N.
South Africa, Tygerberg
South African Medical Research Council
Weyer, Karin
South Africa, Tygerberg
South African Medical Research Council
Fourie, P. B.
South Africa, Pretoria
Medicine in Need Mend
Grewal, Harleen M.S.
Norway, Bergen
Universitetet I Bergen
Statistics
Citations: 112
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1128/JCM.00973-08
ISSN:
00951137
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
South Africa