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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
New enteroviruses, EV-93 and EV-94, associated with acute flaccid paralysis in the Democratic Republic of the Congo
Journal of Medical Virology, Volume 79, No. 4, Year 2007
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Description
Surveillance of acute flaccid paralysis often identifies enteroviruses not typeable by virus neutralization in cell culture. During 2000 and 2001, 186 isolates from 138 children with acute flaccid paralysis in the Democratic Republic of the Congo were sent for typing to the National Reference Centre for Enteroviruses in Lyon, France. The 5′ UTR of the viral genome could be amplified by PCR for 158 isolates from 114 patients. Isolates from 89 patients were neutralizable, and contained non-polio enterovirus types. Seventeen children were infected with more than one entero- or adenovirus; another three were co-infected with both these viruses. Serological typing failed with 19 isolates from 13 (9%) patients. The VP1 region of these strains could be amplified by PCR and sequenced, which revealed that five children were infected with CV-A17, EV-70, EV-76, EV-77, or CV-A13. Two patients were doubly infected, one with CV-A24 and E-9, and another with E-27 and EV-81. Isolates from six children contained strains with divergent VP1 region. The amino acid sequences of these complete VP1 regions diverged ≥28% from published types indicating that they represented two new enterovirus types, tentatively designated EV-93 belonging to HEV-B and EV-94 within HEV-D. The latter enterovirus has in parallel been isolated from sewage in Egypt. In conclusion, there was a high frequency of "untypable" enterovirus isolates from cases with acute flaccid paralysis in the Democratic Republic of the Congo. Six of these were shown to represent two enteroviruses not previously described. © 2007 Wiley-Liss, Inc.
Authors & Co-Authors
Junttila, Nina
Sweden, Solna
Public Health Agency of Sweden
Lévêque, Nicolas
France, Villeurbanne
Université Claude Bernard Lyon 1
Kabue, Jean Pierre
Congo
Institut National de Recherche Biomédicale
Cartet, Gaëlle
France, Villeurbanne
Université Claude Bernard Lyon 1
Mushiya, Fidèle
Congo
Institut National de Recherche Biomédicale
Muyembe-Tamfum, Jean Jacques
Congo
Institut National de Recherche Biomédicale
Trompette, Aurélien
France, Villeurbanne
Université Claude Bernard Lyon 1
Lina, Bruno
France, Villeurbanne
Université Claude Bernard Lyon 1
Magnius, Lars O.
Sweden, Solna
Public Health Agency of Sweden
Chomel, Jean Jacques
France, Villeurbanne
Université Claude Bernard Lyon 1
Norder, Heléne
Sweden, Solna
Public Health Agency of Sweden
France, Villeurbanne
Université Claude Bernard Lyon 1
Statistics
Citations: 79
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1002/jmv.20825
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Maternal And Child Health
Study Locations
Congo
Egypt