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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags
Molecular Ecology Notes, Volume 5, No. 4, Year 2005
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Description
The detection of simple sequence repeats (SSRs) within expressed sequence tags (ESTs) connects potential microsatellite markers with specific genes, generating Type I markers. Using an in silico approach, we identified 1975 SSRs from the Genome Research on Atlantic Salmon Project EST database. We designed primers to amplify 158 SSRs, of which 65 amplified 76 loci (including 11 duplicated loci). Sixty-one of the 76 loci were variable in 24 Atlantic salmon from seven populations, and 96% of these markers also amplify DNA from other salmonids. Functions for 16 of the SSR associated ESTs have been determined, confirming them as Type I markers. © 2005 Blackwell Publishing Ltd.
Authors & Co-Authors
Brown, Gordon D.A.
Canada, Victoria
University of Victoria
Koop, Ben F.
Canada, Victoria
University of Victoria
Statistics
Citations: 23
Authors: 2
Affiliations: 2
Identifiers
Doi:
10.1111/j.1471-8286.2005.01056.x
ISSN:
14718278
Research Areas
Genetics And Genomics